GSBS Student Publications

Title

RPP25 is developmentally regulated in prefrontal cortex and expressed at decreased levels in autism spectrum disorder

Student Author(s)

Hsien-Sung Huang

GSBS Program

Neuroscience

UMMS Affiliation

Department of Psychiatry, Brudnick Neuropsychiatric Research Institute

Date

7-16-2010

Document Type

Article

Medical Subject Headings

Adolescent; Adult; Aged; Animals; Child; Child Development Disorders, Pervasive; Child, Preschool; Chromosomes, Human, Pair 15; Cohort Studies; Female; Gene Expression; Genetic Predisposition to Disease; Humans; Infant, Newborn; Interneurons; Male; Mice; Middle Aged; Prefrontal Cortex; Pregnancy; RNA Precursors; RNA, Transfer; Rats; Ribonuclease P; Transcription, Genetic; Young Adult

Disciplines

Life Sciences | Medicine and Health Sciences | Neuroscience and Neurobiology

Abstract

Dysfunction of cerebral cortex in autism is thought to involve alterations in inhibitory neurotransmission. Here, we screened, in prefrontal cortex (PFC) of 15 subjects diagnosed with autism and 15 matched controls the expression of 44 transcripts that are either preferentially expressed in gamma-aminobutyric acidergic interneurons of the mature cortex or important for the development of inhibitory circuitry. Significant alterations in the autism cohort included decreased expression (-45%) of RPP25 (15q24.1), which is located within the autism susceptibility locus, 15q22-26. RPP25, which encodes the 25 kDa subunit of ribonuclease P involved in tRNA and pre-ribosomal RNA processing, was developmentally regulated in cerebral cortex with peak levels of expression during late fetal development (human) or around birth (mouse). In the PFC, RPP25 chromatin showed high levels of histone H3-lysine 4 trimethylation, an epigenetic mark associated with transcriptional regulation. Unexpectedly, and in contrast to peripheral tissues, levels of RPP25 protein remained undetectable in fetal and adult cerebral cortex. Taken together, these findings suggest a potential role for the RPP25 gene transcript in the neurobiology of developmental brain disorders.

Rights and Permissions

Citation: Autism Res. 2010 Aug;3(4):153-61. Link to article on publisher's site

DOI of Published Version

10.1002/aur.141

Related Resources

Link to Article in PubMed

Journal Title

Autism research : official journal of the International Society for Autism Research

PubMed ID

20632321