GSBS Student Publications

Student Author(s)

Caroline Connor

GSBS Program

Neuroscience

UMMS Affiliation

Department of Biochemistry and Molecular Pharmacology; Program in Bioinformatics and Integrative Biology; Brudnick Neuropsychiatric Research Institute, Department of Psychiatry

Date

7-1-2010

Document Type

Article

Medical Subject Headings

Amino Acid Sequence; Antibodies; *Antibody Specificity; Cells, Cultured; Chromatin; Chromatin Immunoprecipitation; Histones; Methylation; Transcription Initiation Site

Disciplines

Life Sciences | Medicine and Health Sciences | Neuroscience and Neurobiology

Abstract

Antibodies differentiating between the mono-, di- and trimethylated forms of specific histone lysine residues are a critical tool in epigenome research, but show variable specificity, potentially limiting comparisons across studies and between samples. Using trimethyl histone H3 lysine 4 (H3K4me3)-a mark enriched at transcription start sites (TSS) of active genes-as an example, we describe how simple co-incubation with synthetic peptide of the K4me2 modification leads to increased specificity for K4me3 and a much sharper peak distribution proximal to TSS following chromatin immunoprecipitation and massively parallel sequencing (ChIP-Seq).

Rights and Permissions

Citation: Epigenetics. 2010 Jul 1;5(5):392-5. Epub 2010 Jul 1. Link to article on publisher's website

DOI of Published Version

10.4161/epi.5.5.11874

Comments

This is an open access article licensed under a under a Creative Commons Attribution-NonCommercial 3.0 Unported License.

Related Resources

Link to Article in PubMed

Journal Title

Epigenetics : official journal of the DNA Methylation Society

PubMed ID

20458167

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