Department of Biochemistry and Molecular Pharmacology; Program in Bioinformatics and Integrative Biology; Brudnick Neuropsychiatric Research Institute, Department of Psychiatry
Medical Subject Headings
Amino Acid Sequence; Antibodies; *Antibody Specificity; Cells, Cultured; Chromatin; Chromatin Immunoprecipitation; Histones; Methylation; Transcription Initiation Site
Life Sciences | Medicine and Health Sciences | Neuroscience and Neurobiology
Antibodies differentiating between the mono-, di- and trimethylated forms of specific histone lysine residues are a critical tool in epigenome research, but show variable specificity, potentially limiting comparisons across studies and between samples. Using trimethyl histone H3 lysine 4 (H3K4me3)-a mark enriched at transcription start sites (TSS) of active genes-as an example, we describe how simple co-incubation with synthetic peptide of the K4me2 modification leads to increased specificity for K4me3 and a much sharper peak distribution proximal to TSS following chromatin immunoprecipitation and massively parallel sequencing (ChIP-Seq).
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Citation: Epigenetics. 2010 Jul 1;5(5):392-5. Epub 2010 Jul 1. Link to article on publisher's website
Epigenetics : official journal of the DNA Methylation Society
Connor, Caroline M.; Cheung, Iris; Simon, Andrew; Jakovcevski, Mira; Weng, Zhiping; and Akbarian, Schahram, "A simple method for improving the specificity of anti-methyl histone antibodies" (2010). GSBS Student Publications. 1690.