GSBS Student Publications

Title

MutS inhibits RecA-mediated strand exchange with platinated DNA substrates

UMMS Affiliation

Graduate School of Biomedical Sciences; Department of Biochemistry and Molecular Pharmacology

Date

9-18-2004

Document Type

Article

Medical Subject Headings

Adenosine Triphosphatases; Antineoplastic Agents; Bacterial Proteins; Base Pair Mismatch; Cell Survival; Cisplatin; DNA; DNA Adducts; DNA Repair; DNA Repair Enzymes; DNA, Single-Stranded; DNA-Binding Proteins; Escherichia coli; Escherichia coli Proteins; MutS DNA Mismatch-Binding Protein; Nucleic Acid Conformation; Rec A Recombinases; Recombination, Genetic; Site-Specific DNA-Methyltransferase (Adenine-Specific)

Disciplines

Life Sciences | Medicine and Health Sciences

Abstract

Human cell lines and Escherichia coli dam mutants are sensitive to the cytotoxic action of the anticancer agent, cisplatin. Introduction of mutations disabling DNA mismatch repair into these cell lines renders them resistant to the action of this drug. We used RecA-mediated strand exchange between homologous phiX174 molecules, one that was platinated and the other that was unmodified, to show that strand transfer is decreased in a dose-dependent manner. Transfer was severely decreased at 10 adducts per molecule (5,386 bp) and abolished with 24 adducts. At low levels of adduction, addition of MutS to the reaction further decreases the rate and yield in a dose-dependent manner. MutL addition was without effect even in the presence of MutS. The results suggest that although mismatch repair is beneficial for mutation avoidance, its antirecombination activity on inappropriate substrates can be lethal to the cell.

Rights and Permissions

Citation: Proc Natl Acad Sci U S A. 2004 Sep 28;101(39):14174-9. Epub 2004 Sep 16. Link to article on publisher's site

Related Resources

Link to article in PubMed

Journal Title

Proceedings of the National Academy of Sciences of the United States of America

PubMed ID

15375217