Title
Regulated expression of the Escherichia coli dam gene
GSBS Program
Biochemistry & Molecular Pharmacology
UMMS Affiliation
Graduate School of Biomedical Sciences; Department of Biochemistry and Molecular Pharmacology
Date
8-5-2003
Document Type
Article
Medical Subject Headings
ATP-Dependent Proteases; Arabinose; Culture Media; DNA Methylation; Escherichia coli; *Escherichia coli Proteins; *Gene Expression Regulation, Bacterial; Heat-Shock Proteins; Mutation; Promoter Regions (Genetics); *Protease La; Serine Endopeptidases; Site-Specific DNA-Methyltransferase; (Adenine-Specific); Transcription, Genetic
Disciplines
Life Sciences | Medicine and Health Sciences
Abstract
Regulated expression of the Escherichia coli dam gene has been achieved with the araBAD promoter lacking a ribosome binding site. Cultures of dam mutants containing plasmid pMQ430 show no detectable methylation in the absence of arabinose and complete methylation in its presence. Dam methyltransferase is a substrate for the Lon protease.
Rights and Permissions
Citation: J Bacteriol. 2003 Aug;185(16):5012-4.