GSBS Student Publications

Title

Regulated expression of the Escherichia coli dam gene

GSBS Program

Biochemistry & Molecular Pharmacology

UMMS Affiliation

Graduate School of Biomedical Sciences; Department of Biochemistry and Molecular Pharmacology

Date

8-5-2003

Document Type

Article

Medical Subject Headings

ATP-Dependent Proteases; Arabinose; Culture Media; DNA Methylation; Escherichia coli; *Escherichia coli Proteins; *Gene Expression Regulation, Bacterial; Heat-Shock Proteins; Mutation; Promoter Regions (Genetics); *Protease La; Serine Endopeptidases; Site-Specific DNA-Methyltransferase; (Adenine-Specific); Transcription, Genetic

Disciplines

Life Sciences | Medicine and Health Sciences

Abstract

Regulated expression of the Escherichia coli dam gene has been achieved with the araBAD promoter lacking a ribosome binding site. Cultures of dam mutants containing plasmid pMQ430 show no detectable methylation in the absence of arabinose and complete methylation in its presence. Dam methyltransferase is a substrate for the Lon protease.

Rights and Permissions

Citation: J Bacteriol. 2003 Aug;185(16):5012-4.

Related Resources

Link to article in PubMed

Journal Title

Journal of bacteriology

PubMed ID

12897023