Graduate School of Biomedical Sciences; Department of Biochemistry and Molecular Pharmacology
Medical Subject Headings
Adenosine Triphosphatases; Bacterial Proteins; Bacteriophage phi X 174; Base Pair Mismatch; DNA Damage; *DNA Methylation; *DNA Repair; DNA, Viral; DNA-Binding Proteins; Escherichia coli; Escherichia coli Proteins; Mass Spectrometry; Methylnitronitrosoguanidine; MutS DNA Mismatch-Binding Protein; O(6)-Methylguanine-DNA Methyltransferase; Rec A Recombinases; Transcription Factors
Life Sciences | Medicine and Health Sciences
DNA mismatch repair (MMR) sensitizes human and Escherichia coli dam cells to the cytotoxic action of N-methyl-N'-nitro-N-nitrosoguanidine (MNNG) while abrogation of such repair results in drug resistance. In DNA methylated by MNNG, MMR action is the result of MutS recognition of O6-methylguanine base pairs. MutS and Ada methyltransferase compete for the MNNG-induced O6-methylguanine residues, and MMR-induced cytotoxicity is abrogated when Ada is present at higher concentrations than normal. To test the hypothesis that MMR sensitization is due to decreased recombinational repair, we used a RecA-mediated strand exchange assay between homologous phiX174 substrate molecules, one of which was methylated with MNNG. MutS inhibited strand transfer on such substrates in a concentration-dependent manner and its inhibitory effect was enhanced by MutL. There was no effect of these proteins on RecA activity with unmethylated substrates. We quantified the number of O6-methylguanine residues in methylated DNA by HPLC-MS/MS and 5-10 of these residues in phiX174 DNA (5386 bp) were sufficient to block the RecA reaction in the presence of MutS and MutL. These results are consistent with a model in which methylated DNA is perceived by the cell as homeologous and prevented from recombining with homologous DNA by the MMR system.
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Originally published: Nucleic Acids Res. 2005 Jun 22;33(11):3591-7. Link to article on publisher's site
Calmann, Melissa A.; Evans, James E.; and Marinus, Martin G., "MutS inhibits RecA-mediated strand transfer with methylated DNA substrates" (2005). GSBS Student Publications. Paper 167.