Induction of heme oxygenase in intestinal epithelial cells: studies in Caco-2 cell cultures
Biochemistry & Molecular Pharmacology
Graduate School of Biomedical Sciences
Medical Subject Headings
Enzyme Induction; Epithelial Cells; Epithelium; Heme Oxygenase (Decyclizing); Humans; Intestines; Tumor Cells, Cultured
Life Sciences | Medicine and Health Sciences
Enterally administered, heme is a good source of iron in humans and other animals, but the metabolism of heme by enterocytes has not been fully characterized. Caco-2 cells in culture provide a useful model for studying cells that resemble small intestinal epithelium, both morphologically and functionally. In this paper we show that heme oxygenase, the rate-controlling enzyme of heme catabolism, is present in abundance in Caco-2 cells, and that levels of its mRNA and activity can be increased by exposure of the cells to heme or metal ions (cadmium, cobalt). Caco-2 cells also contain biliverdin reductase activity which, in the basal state, is similar to that of heme oxygenase (approximately 40 pmole of product per mg protein per minute); however, when heme oxygenase is induced, biliverdin reductase may become rate-limiting for bilirubin production.
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Citation: Mol Cell Biochem. 1993 Dec 8;129(1):93-8.
Molecular and cellular biochemistry
Cable, Julia W.; Cable, Edward Earl; and Bonkovsky, Herbert L., "Induction of heme oxygenase in intestinal epithelial cells: studies in Caco-2 cell cultures" (1993). GSBS Student Publications. 165.