Temporally- and spatially-regulated transcriptional activity of the nicotinic acetylcholine receptor beta4 subunit gene promoter.
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Authors
Bruschweiler-Li, LeiFuentes Medel, Yuly F.
Scofield, Michael D.
Trang, Ellen B. T.
Binke, Sarah A.
Gardner, Paul D.
Student Authors
Yuly F. Fuentes MedelMichael D. Scofield
Academic Program
Neuroscience; InterdisciplinaryUMass Chan Affiliations
Gardner LabGraduate School of Biomedical Sciences
Neurobiology
Brudnick Neuropsychiatric Research Institute
Psychiatry
Document Type
Journal ArticlePublication Date
2010-03-31Keywords
Receptors, Nicotinic; Gene Expression Regulation; Transcription, GeneticLife Sciences
Medicine and Health Sciences
Metadata
Show full item recordAbstract
Signaling through nicotinic acetylcholine (nACh) receptors underlies a diverse array of behaviors. In order for appropriate signaling to occur via nACh receptors, it is necessary for the genes encoding the receptor subunits to be expressed in a highly regulated temporal and spatial manner. Here we report a transgenic mouse approach to characterize the transcriptional regulation of the gene encoding the nACh receptor beta4 subunit. nACh receptors containing this subunit play critical roles in both the central and peripheral nervous systems. We demonstrate that a 2.3-kilobase pair fragment of the beta4 5'-flanking region is capable of directing reporter gene expression in transgenic animals. Importantly, the transcriptional activity of the promoter region is cell-type-specific and developmentally regulated and overlaps to a great extent with endogenous beta4 mRNA expression. These data indicate that the 2.3-kilobase pair fragment contains transcriptional regulatory elements critical for appropriate beta4 subunit gene expression.Source
Bruschweiler-Li L, Medel YF, Scofield MD, Trang EB, Binke SA, Gardner PD (2010) Temporally- and spatially-regulated transcriptional activity of the nicotinic acetylcholine receptor beta4 subunit gene promoter. Neuroscience 166:864-877.
DOI
10.1016/j.neuroscience.2010.01.026Permanent Link to this Item
http://hdl.handle.net/20.500.14038/33100PubMed ID
20096338Related Resources
ae974a485f413a2113503eed53cd6c53
10.1016/j.neuroscience.2010.01.026