GSBS Student Publications

Student Author(s)

Colin Conine

GSBS Program

Interdisciplinary Graduate Program

UMMS Affiliation

Program in Molecular Medicine

Date

2-23-2010

Document Type

Article

Medical Subject Headings

Animals; Caenorhabditis elegans; Caenorhabditis elegans Proteins; Fertility; Hot Temperature; Male; Mutation; RNA, Small Interfering; RNA-Binding Proteins; Spermatogenesis; Spermatozoa

Disciplines

Life Sciences | Medicine and Health Sciences | Nucleic Acids, Nucleotides, and Nucleosides

Abstract

Gametogenesis is a thermosensitive process in numerous metazoans, ranging from worms to man. In Caenorhabditis elegans, a variety of RNA-binding proteins that associate with germ-line nuage (P granules), including the Piwi-clade argonaute PRG-1, have been implicated in maintaining fertility at elevated temperature. Here we describe the role of two AGO-class paralogs, alg-3 (T22B3.2) and alg-4 (ZK757.3), in promoting thermotolerant male fertility. A rescuing GFP::alg-3 transgene is localized to P granules beginning at the late pachytene stage of male gametogenesis. alg-3/4 double mutants lack a subgroup of small RNAs, the 26G-RNAs which target and appear to down-regulate numerous spermatogenesis-expressed mRNAs. These findings add to a growing number of AGO pathways required for thermotolerant fertility in C. elegans and support a model in which AGOs and their small RNA cofactors function to promote robustness in gene-expression networks.

Rights and Permissions

Conine CC, Batista PJ, Gu W, Claycomb JM, Chavez DA, Shirayama M, Mello CC (2010) The Argonautes ALG-3 and ALG-4 are required for spermatogenesis-specific 26G-RNAs and thermotolerant sperm in Caenorhabditis elegans. PNAS 107:3588-3593. Link to article on publisher's website

DOI of Published Version

10.1073/pnas.0911685107

Related Resources

Link to article in PubMed

Journal Title

Proceedings of the National Academy of Sciences of the United States of America

PubMed ID

20133686

Share

COinS
 
 

To view the content in your browser, please download Adobe Reader or, alternately,
you may Download the file to your hard drive.

NOTE: The latest versions of Adobe Reader do not support viewing PDF files within Firefox on Mac OS and if you are using a modern (Intel) Mac, there is no official plugin for viewing PDF files within the browser window.