Mechanism of synergistic induction of hepatic heme oxygenase by glutethimide and iron: studies in cultured chick embryo liver cells
Biochemistry & Molecular Pharmacology
Graduate School of Biomedical Sciences
Medical Subject Headings
Animals; Blotting, Northern; Blotting, Western; Chick Embryo; Drug Synergism; Enzyme Induction; Ferric Compounds; Glutethimide; Heme Oxygenase (Decyclizing); Heptanoates; Iron; Liver; Mixed Function Oxygenases; Nitrilotriacetic Acid; RNA, Messenger
Life Sciences | Medicine and Health Sciences
Heme oxygenase, the rate controlling enzyme for heme catabolism, is inducible by a variety of treatments, some of which induce by a heme-dependent mechanism and others by a heme-independent mechanism. This work shows that, in cultured chick embryo liver cells, synergistic induction of heme oxygenase by iron, added with the phenobarbital-like drug, glutethimide was heme-dependent. Addition of an inhibitor of heme biosynthesis abolished the synergistic induction of heme oxygenase providing evidence for the heme-dependent mechanism of induction. Glutethimide and iron appeared to induce at the transcriptional level since both heme oxygenase mRNA and protein levels correlate with changes in heme oxygenase activity.
Rights and Permissions
Citation: Biochem Biophys Res Commun. 1990 Apr 16;168(1):176-81.
Biochemical and biophysical research communications
Cable, Edward Earl; Greene, Yvonne J.; Healey, John F.; Evans, Chheng-Orn; and Bonkovsky, Herbert L., "Mechanism of synergistic induction of hepatic heme oxygenase by glutethimide and iron: studies in cultured chick embryo liver cells" (1990). GSBS Student Publications. 161.