GSBS Student Publications

Title

Mechanism of synergistic induction of hepatic heme oxygenase by glutethimide and iron: studies in cultured chick embryo liver cells

GSBS Program

Biochemistry & Molecular Pharmacology

UMMS Affiliation

Graduate School of Biomedical Sciences

Date

4-16-1990

Document Type

Article

Medical Subject Headings

Animals; Blotting, Northern; Blotting, Western; Chick Embryo; Drug Synergism; Enzyme Induction; Ferric Compounds; Glutethimide; Heme Oxygenase (Decyclizing); Heptanoates; Iron; Liver; Mixed Function Oxygenases; Nitrilotriacetic Acid; RNA, Messenger

Disciplines

Life Sciences | Medicine and Health Sciences

Abstract

Heme oxygenase, the rate controlling enzyme for heme catabolism, is inducible by a variety of treatments, some of which induce by a heme-dependent mechanism and others by a heme-independent mechanism. This work shows that, in cultured chick embryo liver cells, synergistic induction of heme oxygenase by iron, added with the phenobarbital-like drug, glutethimide was heme-dependent. Addition of an inhibitor of heme biosynthesis abolished the synergistic induction of heme oxygenase providing evidence for the heme-dependent mechanism of induction. Glutethimide and iron appeared to induce at the transcriptional level since both heme oxygenase mRNA and protein levels correlate with changes in heme oxygenase activity.

Rights and Permissions

Citation: Biochem Biophys Res Commun. 1990 Apr 16;168(1):176-81.

Related Resources

Link to article in PubMed

Journal Title

Biochemical and biophysical research communications

PubMed ID

2327996