A Tyramine-Gated Chloride Channel Coordinates Distinct Motor Programs of a Caenorhabditis elegans Escape Response
Department of Neurobiology; Alkema Lab; Francis Lab
Medical Subject Headings
Adrenergic Uptake Inhibitors; Analysis of Variance; Animals; Animals, Genetically Modified; Behavior, Animal; Caenorhabditis elegans; Caenorhabditis elegans Proteins; Chloride Channels; Dose-Response Relationship, Drug; Electric Stimulation; Escape Reaction; Gene Expression; Green Fluorescent Proteins; Head Movements; Ion Channel Gating; Locomotion; Membrane Potentials; Mutation; Neck Muscles; Oocytes; Patch-Clamp Techniques; Physical Stimulation; Sequence Analysis, Protein; Tyramine; Xenopus laevis
Neuroscience and Neurobiology
A key feature of escape responses is the fast translation of sensory information into a coordinated motor output. In C. elegans, anterior touch initiates a backward escape response in which lateral head movements are suppressed. Here, we show that tyramine inhibits head movements and forward locomotion through the activation of a tyramine-gated chloride channel, LGC-55. lgc-55 mutant animals have defects in reversal behavior and fail to suppress head oscillations in response to anterior touch. lgc-55 is expressed in neurons and muscle cells that receive direct synaptic inputs from tyraminergic motor neurons. Therefore, tyramine can act as a classical inhibitory neurotransmitter. Activation of LGC-55 by tyramine coordinates the output of two distinct motor programs, locomotion and head movements that are critical for a C. elegans escape response.
Rights and Permissions
Pirri, J.K., McPherson, A.D., Donnelly, J.L., Francis, M.M., and Alkema, M.J. (2009). A Tyramine-Gated Chloride Channel Coordinates Distinct Motor Programs of a Caenorhabditis elegans Escape Response. Neuron. 62, 528-536. Link to article on publisher's website
Pirri, Jennifer K.; McPherson, Adam D.; Donnelly, Jamie L.; Francis, Michael M.; and Alkema, Mark J., "A Tyramine-Gated Chloride Channel Coordinates Distinct Motor Programs of a Caenorhabditis elegans Escape Response" (2009). GSBS Student Publications. Paper 1594.