A simplified miRNA-based gene silencing method for Drosophila melanogaster
Graduate School of Biomedical Sciences; Department of Molecular and Cell Biology
Medical Subject Headings
Animals; Cell Line; Drosophila Proteins; Drosophila melanogaster; *Genetic Techniques; Genetic Vectors; MicroRNAs; *RNA Interference; RNA-Binding Proteins; Wing
Life Sciences | Medicine and Health Sciences
MicroRNA-based RNA interference is commonly used to produce loss-of-function phenotypes in mammalian systems, but is used only sparingly in invertebrates such as Caenorhabditis elegans and Drosophila melanogaster. Here, we evaluate this method in transgenic strains of D. melanogaster and cultured S2 cells. High throughput-ready expression vectors were developed that permit rapid cloning of synthetic hairpin RNAs. As proof of concept, this method was used for the efficient silencing of dpp gene activity in the adult wing, and the analysis of the general RNA Polymerase II (Pol II) elongation factor, Nelf-E.
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Citation: Dev Biol. 2008 Sep 15;321(2):482-90. Epub 2008 Jun 19. Link to article on publisher's site
Haley, Benjamin; Hendrix, David A.; Trang, Vinh; and Levine, Michael, "A simplified miRNA-based gene silencing method for Drosophila melanogaster" (2008). GSBS Student Publications. 1532.