GSBS Student Publications

Title

A simplified miRNA-based gene silencing method for Drosophila melanogaster

UMMS Affiliation

Graduate School of Biomedical Sciences; Department of Molecular and Cell Biology

Date

7-5-2008

Document Type

Article

Medical Subject Headings

Animals; Cell Line; Drosophila Proteins; Drosophila melanogaster; *Genetic Techniques; Genetic Vectors; MicroRNAs; *RNA Interference; RNA-Binding Proteins; Wing

Disciplines

Life Sciences | Medicine and Health Sciences

Abstract

MicroRNA-based RNA interference is commonly used to produce loss-of-function phenotypes in mammalian systems, but is used only sparingly in invertebrates such as Caenorhabditis elegans and Drosophila melanogaster. Here, we evaluate this method in transgenic strains of D. melanogaster and cultured S2 cells. High throughput-ready expression vectors were developed that permit rapid cloning of synthetic hairpin RNAs. As proof of concept, this method was used for the efficient silencing of dpp gene activity in the adult wing, and the analysis of the general RNA Polymerase II (Pol II) elongation factor, Nelf-E.

Rights and Permissions

Citation: Dev Biol. 2008 Sep 15;321(2):482-90. Epub 2008 Jun 19. Link to article on publisher's site

DOI of Published Version

10.1016/j.ydbio.2008.06.015

Related Resources

Link to Article in PubMed

Journal Title

Developmental biology

PubMed ID

18598689