GSBS Student Publications

Title

Three-dimensional sub-100 nm resolution fluorescence microscopy of thick samples

UMMS Affiliation

Graduate School of Biomedical Sciences; Department of Biochemistry and Molecular Pharmacology

Date

5-13-2008

Document Type

Article

Medical Subject Headings

Biophysics; Fluorescein; Fluorescent Dyes; Image Enhancement; Image Interpretation, Computer-Assisted; Imaging, Three-Dimensional; Lasers; Light; Microscopy; Microscopy, Fluorescence; Software

Disciplines

Life Sciences | Medicine and Health Sciences

Abstract

Imaging volumes as thick as whole cells at three-dimensional (3D) super-resolution is required to reveal unknown features of cellular organization. We report a light microscope that generates images with translationally invariant 30 x 30 x 75 nm resolution over a depth of several micrometers. This method, named biplane (BP) FPALM, combines a double-plane detection scheme with fluorescence photoactivation localization microscopy (FPALM) enabling 3D sub-diffraction resolution without compromising speed or sensitivity.

Rights and Permissions

Citation: Nat Methods. 2008 Jun;5(6):527-9. Epub 2008 May 11. Link to article on publisher's site

DOI of Published Version

10.1038/nmeth.1211

Related Resources

Link to Article in PubMed

Journal Title

Nature methods

PubMed ID

18469823