Three-dimensional sub-100 nm resolution fluorescence microscopy of thick samples
Graduate School of Biomedical Sciences; Department of Biochemistry and Molecular Pharmacology
Medical Subject Headings
Biophysics; Fluorescein; Fluorescent Dyes; Image Enhancement; Image Interpretation, Computer-Assisted; Imaging, Three-Dimensional; Lasers; Light; Microscopy; Microscopy, Fluorescence; Software
Life Sciences | Medicine and Health Sciences
Imaging volumes as thick as whole cells at three-dimensional (3D) super-resolution is required to reveal unknown features of cellular organization. We report a light microscope that generates images with translationally invariant 30 x 30 x 75 nm resolution over a depth of several micrometers. This method, named biplane (BP) FPALM, combines a double-plane detection scheme with fluorescence photoactivation localization microscopy (FPALM) enabling 3D sub-diffraction resolution without compromising speed or sensitivity.
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Citation: Nat Methods. 2008 Jun;5(6):527-9. Epub 2008 May 11. Link to article on publisher's site
Juette, Manuel F.; Gould, Travis J.; Lessard, Mark D.; Mlodzianoski, Michael J.; Nagpure, Bhupendra S.; Bennett, Brian Thomas; Hess, Samuel T.; and Bewersdorf, Jorg, "Three-dimensional sub-100 nm resolution fluorescence microscopy of thick samples" (2008). GSBS Student Publications. 1500.