GSBS Student Publications

Title

In situ nuclear organization of regulatory machinery

UMMS Affiliation

Department of Cell Biology

Date

5-9-2008

Document Type

Article

Medical Subject Headings

Animals; *Cell Culture Techniques; Cell Cycle; Cell Nucleus; Cells, Cultured; Chromosomes; Fluorescence Recovery After Photobleaching; *Gene Expression Regulation; Humans; Intermediate Filaments; Microscopy, Fluorescence

Disciplines

Cell Biology | Life Sciences | Medicine and Health Sciences

Abstract

Regulatory machinery for gene expression, replication, and repair are architecturally organized in nuclear microenvironments. This compartmentalization provides threshold concentrations of macromolecules for the organization and assembly of regulatory complexes for combinatorial control. A mechanistic under standing of biological control requires the combined application of molecular, cellular, biochemical, and in vivo genetic approaches. This chapter provides methodologies to characterize nuclear organization of regulatory machinery by in situ immunofluorescence microscopy.

Rights and Permissions

Citation: Methods Mol Biol. 2008;455:239-59. Link to article on publisher's site

DOI of Published Version

10.1007/978-1-59745-104-8_17

Related Resources

Link to Article in PubMed

Journal Title

Methods in molecular biology (Clifton, N.J.)

PubMed ID

18463823