GSBS Student Publications


In situ nuclear organization of regulatory machinery

UMMS Affiliation

Department of Cell Biology



Document Type


Medical Subject Headings

Animals; *Cell Culture Techniques; Cell Cycle; Cell Nucleus; Cells, Cultured; Chromosomes; Fluorescence Recovery After Photobleaching; *Gene Expression Regulation; Humans; Intermediate Filaments; Microscopy, Fluorescence


Cell Biology | Life Sciences | Medicine and Health Sciences


Regulatory machinery for gene expression, replication, and repair are architecturally organized in nuclear microenvironments. This compartmentalization provides threshold concentrations of macromolecules for the organization and assembly of regulatory complexes for combinatorial control. A mechanistic under standing of biological control requires the combined application of molecular, cellular, biochemical, and in vivo genetic approaches. This chapter provides methodologies to characterize nuclear organization of regulatory machinery by in situ immunofluorescence microscopy.

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Citation: Methods Mol Biol. 2008;455:239-59. Link to article on publisher's site

DOI of Published Version


Related Resources

Link to Article in PubMed

Journal Title

Methods in molecular biology (Clifton, N.J.)

PubMed ID