In situ nuclear organization of regulatory machinery
Department of Cell Biology
Medical Subject Headings
Animals; *Cell Culture Techniques; Cell Cycle; Cell Nucleus; Cells, Cultured; Chromosomes; Fluorescence Recovery After Photobleaching; *Gene Expression Regulation; Humans; Intermediate Filaments; Microscopy, Fluorescence
Cell Biology | Life Sciences | Medicine and Health Sciences
Regulatory machinery for gene expression, replication, and repair are architecturally organized in nuclear microenvironments. This compartmentalization provides threshold concentrations of macromolecules for the organization and assembly of regulatory complexes for combinatorial control. A mechanistic under standing of biological control requires the combined application of molecular, cellular, biochemical, and in vivo genetic approaches. This chapter provides methodologies to characterize nuclear organization of regulatory machinery by in situ immunofluorescence microscopy.
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Citation: Methods Mol Biol. 2008;455:239-59. Link to article on publisher's site
Methods in molecular biology (Clifton, N.J.)
Pockwinse, Shirwin M.; Zaidi, Sayyed K.; Medina, Ricardo F.; Bakshi, Rachit; Kota, Krishna P.; Ali, Syed A.; Young, Daniel W.; Nickerson, Jeffrey A.; Javed, Amjad; Montecino, Martin A.; Van Wijnen, Andre J.; Lian, Jane B.; Stein, Janet L.; and Stein, Gary S., "In situ nuclear organization of regulatory machinery" (2008). GSBS Student Publications. 1499.