GSBS Student Publications

Title

Apoptosis-induced inhibition of CD1d-mediated antigen presentation: different roles for caspases and signal transduction pathways

UMMS Affiliation

Graduate School of Biomedical Sciences; Department of Microbiology and Immunology

Date

3-19-2008

Document Type

Article

Medical Subject Headings

Animals; Antigen Presentation; Antigens, CD1; Antigens, CD1d; Apoptosis; Camptothecin; Caspases; Cells, Cultured; Ceramides; Coculture Techniques; Female; Galactosylceramides; Meperidine; Mice; Mice, Inbred C57BL; Protein Kinase C-delta; Signal Transduction; p38 Mitogen-Activated Protein Kinases

Disciplines

Life Sciences | Medicine and Health Sciences

Abstract

The stimulation of programmed cell death can either enhance or inhibit antigen presentation by classic major histocompatibility complex molecules. In the current study, we report that the induction of apoptosis by topoisomerase I inhibition or elevation of intracellular ceramide levels substantially impairs CD1d-mediated antigen presentation. In the former case, such a reduction occurred via the regulation of both the p38 mitogen-activated protein kinases and protein kinase C delta signal transduction pathways as well as the caspase cascade, whereas the latter was p38-(but not caspase)-dependent. Confocal microscopic analysis showed an altered intracellular distribution of CD1d following the inhibition topoisomerase I or by an increase in intracellular ceramide levels, that was prevented by p38 and caspase inhibitors. Thus, the induction of apoptosis in antigen presenting cells severely compromises CD1d-mediated antigen presentation by multiple mechanisms.

Rights and Permissions

Citation: Immunology. 2008 Sep;125(1):80-90. Epub 2008 Mar 14. Link to article on publisher's site

DOI of Published Version

10.1111/j.1365-2567.2008.02823.x

Related Resources

Link to Article in PubMed

Journal Title

Immunology

PubMed ID

18346153