The presence of JAK2V617F in primary myelofibrosis or its allele burden in polycythemia vera predicts chemosensitivity to hydroxyurea
Graduate School of Biomedical Sciences; Department of Biochemistry and Molecular Biology; Program in Molecular Medicine
Medical Subject Headings
Adult; Age Factors; Aged; Alleles; Cohort Studies; DNA Mutational Analysis; Drug Resistance; Female; Humans; Hydroxyurea; Janus Kinase 2; Male; Middle Aged; *Mutation, Missense; Platelet Count; *Point Mutation; Polycythemia Vera; Primary Myelofibrosis; Treatment Outcome
Life Sciences | Medicine and Health Sciences
JAK2V617F-positive patients with essential thrombocythemia, as opposed to their mutation-negative counterparts, require lower doses of hydroxyurea (HU) for control of their platelet count. In the current study, we looked for predictors of HU response in 69 patients with primary myelofibrosis (PMF) and 56 with polycythemia vera (PV). JAK2V617F analysis was performed on bone marrow-derived DNA obtained at or near the time of diagnosis. HU response in PMF was associated with a shorter disease duration (P = 0.008), absence of previous therapy (P = 0.01), older age at diagnosis (P = 0.009), and presence of JAK2V617F (P = 0.02). On multivariable analysis, only the latter retained its significance (48% vs. 8% response in mutation positive vs. negative cases). In PV, JAK2V617F allele burden correlated directly with HU response (P = 0.05) and inversely with daily HU dose in responding patients (P = 0.02). The current study suggests that JAK2V617F presence identifies PMF patients who are likely to respond to HU therapy, and information on its allele burden helps in assigning the optimal starting dose in individual patients with PV.
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Citation: Am J Hematol. 2008 May;83(5):363-5. Link to article on publisher's site
American journal of hematology
Sirhan, Shireen; Lasho, Terra L.; Hanson, Curtis A.; Mesa, Ruben A.; Pardanani, Animesh Dev; and Tefferi, Ayalew, "The presence of JAK2V617F in primary myelofibrosis or its allele burden in polycythemia vera predicts chemosensitivity to hydroxyurea" (2008). GSBS Student Publications. 1448.