Polyfunctional T cell responses are a hallmark of HIV-2 infection
MRC Human Immunology Unit
Medical Subject Headings
CD4-Positive T-Lymphocytes; CD8-Positive T-Lymphocytes; Cytokines; Dose-Response Relationship, Immunologic; Epitopes, T-Lymphocyte; Flow Cytometry; HIV Infections; HIV-1; HIV-2; Humans; Immunophenotyping; T-Lymphocytes
Life Sciences | Medicine and Health Sciences
HIV-2 is distinguished clinically and immunologically from HIV-1 infection by delayed disease progression and maintenance of HIV-specific CD4(+) T cell help in most infected subjects. Thus, HIV-2 provides a unique natural human model in which to investigate correlates of immune protection against HIV disease progression. Here, we report a detailed assessment of the HIV-2-specific CD4(+) and CD8(+) T cell response compared to HIV-1, using polychromatic flow cytometry to assess the quality of the HIV-specific T cell response by measuring IFN-gamma, IL-2, TNF-alpha, MIP-1beta, and CD107a mobilization (degranulation) simultaneously following Gag peptide stimulation. We find that HIV-2-specific CD4(+) and CD8(+) T cells are more polyfunctional that those specific for HIV-1 and that polyfunctional HIV-2-specific T cells produce more IFN-gamma and TNF-alpha on a per-cell basis than monofunctional T cells. Polyfunctional HIV-2-specific CD4(+) T cells were generally more differentiated and expressed CD57, while there was no association between function and phenotype in the CD8(+) T cell fraction. Polyfunctional HIV-specific T cell responses are a hallmark of non-progressive HIV-2 infection and may be related to good clinical outcome in this setting.
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Citation: Eur J Immunol. 2008 Feb;38(2):350-63. Link to article on publisher's site
Duvall, Melody G.; Precopio, Melissa Lynn; Ambrozak, David A.; Jaye, Assan; McMichael, Andrew J.; Whittle, Hilton C.; Roederer, Mario; Rowland-Jones, Sarah L.; and Koup, Richard A., "Polyfunctional T cell responses are a hallmark of HIV-2 infection" (2008). GSBS Student Publications. Paper 1431.