GSBS Student Publications

Title

Alu elements mediate MYB gene tandem duplication in human T-ALL

UMMS Affiliation

Department of Pediatric Oncology; Department of Molecular Genetics and Microbiology

Date

12-12-2007

Document Type

Article

Medical Subject Headings

Alu Elements; Base Sequence; *Gene Duplication; Humans; In Situ Hybridization, Fluorescence; Leukemia-Lymphoma, Adult T-Cell; Models, Biological; Models, Genetic; Molecular Sequence Data; Nucleic Acid Hybridization; Polymerase Chain Reaction; Proto-Oncogene Proteins c-myb; Recombination, Genetic

Disciplines

Life Sciences | Medicine and Health Sciences

Abstract

Recent studies have demonstrated that the MYB oncogene is frequently duplicated in human T cell acute lymphoblastic leukemia (T-ALL). We find that the human MYB locus is flanked by 257-bp Alu repeats and that the duplication is mediated somatically by homologous recombination between the flanking Alu elements on sister chromatids. Nested long-range PCR analysis indicated a low frequency of homologous recombination leading to MYB tandem duplication in the peripheral blood mononuclear cells of approximately 50% of healthy individuals, none of whom had a MYB duplication in the germline. We conclude that Alu-mediated MYB tandem duplication occurs at low frequency during normal thymocyte development and is clonally selected during the molecular pathogenesis of human T-ALL.

Rights and Permissions

Citation: J Exp Med. 2007 Dec 24;204(13):3059-66. Epub 2007 Dec 10. Link to article on publisher's site

DOI of Published Version

10.1084/jem.20071637

Related Resources

Link to Article in PubMed

Journal Title

The Journal of experimental medicine

PubMed ID

18070937