GSBS Student Publications

The histone gene cell cycle regulator HiNF-P is a unique zinc finger transcription factor with a novel conserved auxiliary DNA-binding motif

Ricardo F. Medina, University of Massachusetts Medical School
Timothy F. Buck, University of Massachusetts Medical School
Sayyed K. Zaidi, University of Massachusetts Medical School
Angela Miele, University of Massachusetts Medical School
Jane B. Lian, University of Massachusetts Medical School
Janet L. Stein, University of Massachusetts Medical School
Andre J. Van Wijnen, University of Massachusetts Medical School
Gary S. Stein, University of Massachusetts Medical School

Document Type Article

Abstract

Accumulation of histone proteins is necessary for packaging of replicated DNA during the S phase of the cell cycle. Different mechanisms operate to regulate histone protein levels, and induction of human histone gene expression at the G1-S phase transition plays a critical role. The zinc finger HiNF-P and coactivator p220 (NPAT) proteins are key regulators of histone gene expression. Here, we describe a novel HiNF-P-specific conserved region (PSCR) located within the C-terminus that is present in HiNF-P homologues of all metazoan species that have been examined. The PSCR motif is required for activation of histone H4 gene transcription and contributes to DNA binding of HiNF-P. Thus, the PSCR module represents an auxiliary DNA-binding determinant that plays a critical role in mediating histone gene expression during the cell cycle and defines HiNF-P as a unique cell cycle regulatory member of the zinc finger transcription factor family.