Title

Connective tissue growth factor (CTGF/CCN2) is a downstream mediator for TGF-beta1-induced extracellular matrix production in osteoblasts

UMMS Affiliation

Graduate School of Biomedical Sciences; Department of Cell Biology

Date

11-30-2006

Document Type

Article

Medical Subject Headings

Animals; Cell Differentiation; Cell Proliferation; Cells, Cultured; Connective Tissue Growth Factor; Cyclins; Extracellular Matrix; Extracellular Matrix Proteins; Gene Expression Regulation; Immediate-Early Proteins; Intercellular Signaling Peptides and Proteins; Osteoblasts; RNA, Small Interfering; Rats; Transforming Growth Factor beta1

Disciplines

Life Sciences | Medicine and Health Sciences

Abstract

Connective tissue growth factor (CTGF/CCN2) is a cysteine-rich, extracellular matrix (ECM) protein that acts as an anabolic growth factor to regulate osteoblast differentiation and function. Recent studies have identified CTGF as a downstream effector of transforming growth factor-beta1 (TGF-beta1) for certain functions in specific cell types. In this study, we examined the role of CTGF as a downstream mediator of TGF-beta1-induced ECM production and cell growth in osteoblasts. Using primary cultures, we demonstrated that TGF-beta1 is a potent inducer of CTGF expression in osteoblasts, and that this induction occurred at all stages of osteoblast differentiation from the proliferative through mineralization stages. TGF-beta1 treatment of osteoblasts increased the expression and synthesis of the ECM components, collagen and fibronectin. When CTGF-specific siRNA was used to prevent TGF-beta1 induction of CTGF expression, it also inhibited collagen and fibronectin production, thereby demonstrating the requirement of CTGF for their up-regulation. To examine the effects of TGF-beta1 on osteoblast cell growth, cultures were treated with TGF-beta1 during the proliferative stage. Cell number was significantly reduced and the cells exhibited a decrease in G1 cyclin expression, consistent with TGF-beta1-induced cell-cycle arrest. Cultures transfected with CTGF siRNA prior to TGF-beta1 treatment showed an even greater reduction in cell number, suggesting that TGF-beta1-induced growth arrest is independent of CTGF in osteoblasts. Collectively, these data demonstrate for the first time that CTGF is an essential downstream mediator for TGF-beta1-induced ECM production in osteoblasts, but these two growth factors function independently regarding their opposing effects on osteoblast proliferation.

Rights and Permissions

Citation: J Cell Physiol. 2007 Mar;210(3):843-52. Link to article on publisher's site

Related Resources

Link to Article in PubMed

PubMed ID

17133352