GSBS Student Publications

Title

Mus81, Rhp51(Rad51) and Rqh1 Form an Epistatic Pathway Required for the S-phase DNA Damage Checkpoint

UMMS Affiliation

Graduate School of Biomedical Sciences; Department of Biochemistry and Molecular Pharmacology

Date

11-28-2008

Document Type

Article

Disciplines

Life Sciences | Medicine and Health Sciences

Abstract

Monitoring Editor: Orna Cohen-Fix The S-phase DNA damage checkpoint slows the rate of DNA synthesis in response to damage during replication. In the fission yeast Schizosaccharomyces pombe, Cds1, the S-phase specific checkpoint effector kinase, is required for checkpoint signaling and replication slowing; upon treatment with the alkylating agent MMS, cds1Delta mutants display a complete checkpoint defect. We have identified proteins downstream of Cds1 required for checkpoint-dependant slowing, including the structure-specific endonuclease Mus81 and the helicase Rqh1, which are implicated in replication fork stability and the negative regulation of recombination. Removing Rhp51, the Rad51 recombinase homolog, suppresses the slowing defect of rqh1Delta mutants, but not that of mus81Delta mutant, defining an epistatic pathway in which mus81 is epistatic to rhp51 and rhp51 is epistatic to rqh1. We propose that restraining recombination is required for the slowing of replication in response to DNA damage.

Rights and Permissions

Citation: Mol Biol Cell. 2008 Nov 26. Link to article on publisher's site

DOI of Published Version

10.1091/mbc.E08-08-0798

Related Resources

Link to Article in PubMed

Journal Title

Molecular biology of the cell

PubMed ID

19037101