GSBS Student Publications

Title

Lymphocytic choriomeningitis virus (LCMV): propagation, quantitation, and storage

UMMS Affiliation

Graduate School of Biomedical Sciences; Department of Pathology; Department of Molecular Genetics and Microbiology

Date

9-5-2008

Document Type

Article

Medical Subject Headings

Animals; Cell Line; Cercopithecus aethiops; Cricetinae; Cryopreservation; *Culture Techniques; Lymphocytic choriomeningitis virus; Occupational Health; Plaque Assay; RNA, Viral; Reverse Transcriptase Polymerase Chain Reaction; Vero Cells; Virology; Virus Cultivation

Disciplines

Life Sciences | Medicine and Health Sciences

Abstract

Lymphocytic choriomeningitis virus (LCMV) is an enveloped, ambisense RNA virus and the prototypic virus of the arenavirus group. It can cause viral meningitis and other ailments in humans, but its natural host is the mouse. The LCMV/mouse model has been useful for examining mechanisms of viral persistence and the basic concepts of virus-induced immunity and immunopathology. This unit discusses strain differences and biosafety containment issues for LCMV. Recommendations are made for techniques for propagating LCMV to high titers to quantify it by plaque assay and PCR techniques and to preserve its infectivity by appropriate storage.

Rights and Permissions

Citation: Curr Protoc Microbiol. 2008 Feb;Chapter 15:Unit 15A.1. Link to article on publisher's site

DOI of Published Version

10.1002/9780471729259.mc15a01s8

Related Resources

Link to Article in PubMed

Journal Title

Current protocols in microbiology

PubMed ID

18770534