Lymphocytic choriomeningitis virus (LCMV): propagation, quantitation, and storage
Graduate School of Biomedical Sciences; Department of Pathology; Department of Molecular Genetics and Microbiology
Medical Subject Headings
Animals; Cell Line; Cercopithecus aethiops; Cricetinae; Cryopreservation; *Culture Techniques; Lymphocytic choriomeningitis virus; Occupational Health; Plaque Assay; RNA, Viral; Reverse Transcriptase Polymerase Chain Reaction; Vero Cells; Virology; Virus Cultivation
Life Sciences | Medicine and Health Sciences
Lymphocytic choriomeningitis virus (LCMV) is an enveloped, ambisense RNA virus and the prototypic virus of the arenavirus group. It can cause viral meningitis and other ailments in humans, but its natural host is the mouse. The LCMV/mouse model has been useful for examining mechanisms of viral persistence and the basic concepts of virus-induced immunity and immunopathology. This unit discusses strain differences and biosafety containment issues for LCMV. Recommendations are made for techniques for propagating LCMV to high titers to quantify it by plaque assay and PCR techniques and to preserve its infectivity by appropriate storage.
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Citation: Curr Protoc Microbiol. 2008 Feb;Chapter 15:Unit 15A.1. Link to article on publisher's site
DOI of Published Version
Current protocols in microbiology
Welsh, Raymond M. and Seedhom, Mina O., "Lymphocytic choriomeningitis virus (LCMV): propagation, quantitation, and storage" (2008). GSBS Student Publications. 1383.