Influenza A virus matrix protein 1-specific human CD8+ T-cell response induced in trivalent inactivated vaccine recipients
Graduate School of Biomedical Sciences; Center for Infectious Disease and Vaccine Research
Medical Subject Headings
CD4-Positive T-Lymphocytes; CD8-Positive T-Lymphocytes; Cell Line; Epitopes, T-Lymphocyte; HLA-A2 Antigen; Humans; Influenza A Virus, H1N1 Subtype; Influenza A Virus, H3N2 Subtype; Influenza Vaccines; Influenza, Human; Vaccines, Inactivated; Viral Matrix Proteins
Life Sciences | Medicine and Health Sciences
Among 17 HLA-A2-positive healthy adults, CD8+ T-cell responses against an HLA-A2-restricted matrix protein 1 (M1) epitope increased after immunization with trivalent inactivated influenza vaccine (TIV) in two individuals. The presence of M1 in TIV was confirmed by Western blotting. T-cell cytotoxicity assays showed that TIV is processed and the epitope is presented by antigen-presenting cells to an M1 epitope-specific CD8+ T-cell line for specific lysis. These data show that TIV, which is formulated to contain surface glycoproteins to induce serotype-specific antibody responses, also contains M1, capable of inducing subtype cross-reactive CD8+ T-cell responses in some vaccinees.
Rights and Permissions
Citation: J Virol. 2008 Sep;82(18):9283-7. Epub 2008 Jul 9. Link to article on publisher's site
Journal of virology
Terajima, Masanori; Cruz, John; Leporati, Anita M.; Orphin, Laura; Babon, Jenny Aurielle B.; Co, Mary Dawn T.; Pazoles, Pamela P.; Jameson, Julie Marie; and Ennis, Francis A., "Influenza A virus matrix protein 1-specific human CD8+ T-cell response induced in trivalent inactivated vaccine recipients" (2008). GSBS Student Publications. 1380.