Vaccinia peptides eluted from HLA-DR1 isolated from virus-infected cells are recognized by CD4+ T cells from a vaccinated donor
Graduate School of Biomedical Sciences; Department of Pathology; Department of Biochemistry and Molecular Pharmacology; Center for Infectious Disease and Vaccine Research
Medical Subject Headings
Amino Acid Sequence; CD4-Positive T-Lymphocytes; Cell Line, Tumor; Chromatography, Liquid; Epitopes, T-Lymphocyte; HLA-DR1 Antigen; Humans; Molecular Sequence Data; Peptides; Smallpox Vaccine; Tandem Mass Spectrometry; Vaccination; Vaccinia virus; Viral Proteins
Life Sciences | Medicine and Health Sciences
Class II MHC proteins bind peptides and present them to CD4 (+) T cells as part of the immune system's surveillance of bodily tissues for foreign and pathogenic material. Antigen processing and presentation pathways have been characterized in detail in normal cells, but there is little known about the actual viral peptides that are presented to CD4 (+) T cells that signal infection. In this study, two-dimensional LC-MS/MS was used to identify vaccinia virus-derived peptides among the hundreds to thousands of peptide antigens bound to the human class II MHC protein HLA-DR1 on the surface of vaccinia virus-infected cells. The peptides, derived from the I6L, D6R, and A10L viral proteins, were 15 residues in length, bound efficiently to HLA-DR1 as synthetic peptides, and were recognized by vaccinia-specific CD4 (+) T cells obtained from an immunized donor.
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Citation: J Proteome Res. 2008 Jul;7(7):2703-11. Epub 2008 May 29. Link to article on publisher's site
Journal of proteome research
Strug, Iwona; Calvo-Calle, J. Mauricio; Green, Karin M.; Cruz, John; Ennis, Francis A.; Evans, James E.; and Stern, Lawrence J., "Vaccinia peptides eluted from HLA-DR1 isolated from virus-infected cells are recognized by CD4+ T cells from a vaccinated donor" (2008). GSBS Student Publications. 1363.