A Rad51 presynaptic filament is sufficient to capture nucleosomal homology during recombinational repair of a DNA double-strand break
Graduate School of Biomedical Sciences; Program in Molecular Medicine
Medical Subject Headings
Binding Sites; Chromatin; *DNA Damage; *DNA Repair; DNA, Fungal; Histones; Humans; Kinetics; Nucleosomes; Peptide Fragments; Rad51 Recombinase; *Recombination, Genetic; Saccharomyces cerevisiae; Saccharomyces cerevisiae Proteins
Life Sciences | Medicine and Health Sciences
Repair of chromosomal DNA double-strand breaks by homologous recombination is essential for cell survival and genome stability. Within eukaryotic cells, this repair pathway requires a search for a homologous donor sequence and a subsequent strand invasion event on chromatin fibers. We employ a biotin-streptavidin minichromosome capture assay to show that yRad51 or hRad51 presynaptic filaments are sufficient to locate a homologous sequence and form initial joints, even on the surface of a nucleosome. Furthermore, we present evidence that the Rad54 chromatin-remodeling enzyme functions to convert these initial metastable products of the homology search to a stable joint molecule that is competent for subsequent steps of the repair process. Thus, contrary to popular belief, nucleosomes do not pose a potent barrier for successful recognition and capture of homology by an invading presynaptic filament.
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Citation: Mol Cell. 2008 Jun 20;30(6):803-10. Link to article on publisher's site
Sinha, Manisha and Peterson, Craig L., "A Rad51 presynaptic filament is sufficient to capture nucleosomal homology during recombinational repair of a DNA double-strand break" (2008). GSBS Student Publications. 1361.