Transcription factor assembly on the nicotinic receptor beta4 subunit gene promoter
Graduate School of Biomedical Sciences; Department of Psychiatry
Medical Subject Headings
Animals; Blotting, Northern; Blotting, Western; Brain; Cell Line; *Gene Expression Regulation; Immunoprecipitation; Mice; Neurons; Oligonucleotide Array Sequence Analysis; Polymerase Chain Reaction; Promoter Regions, Genetic; Rats; Receptors, Nicotinic; Transcription Factors; Transcription, Genetic
Life Sciences | Medicine and Health Sciences
Nicotinic acetylcholine receptors are involved in a plethora of fundamental biological processes ranging from muscle contraction to formation of memories. The receptors are pentameric proteins whose subunits are encoded by distinct genes. Subunit composition of a mature nicotinic receptor is governed in part by the transcriptional regulation of each subunit gene. Here, using chromatin immunoprecipitation assays, we report the interaction of the transcription factors Sp1, Sp3, c-Jun and Sox10 with the beta4 subunit gene promoter in neuronal-like cell lines and rodent brain tissue. Our results corroborate previous in-vitro data demonstrating that these transcription factors interact with the beta4 promoter. Taken together, these data suggest that Sp1, Sp3, c-Jun and Sox10 regulate expression of the beta4 subunit gene in the mammalian brain.
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Citation: Neuroreport. 2008 Apr 16;19(6):687-90. Link to article on publisher's site
Scofield, Michael D.; Bruschweiler-Li, Lei; Mou, Zhongming; and Gardner, Paul D., "Transcription factor assembly on the nicotinic receptor beta4 subunit gene promoter" (2008). GSBS Student Publications. 1359.