GSBS Student Publications

Title

Regulation of WRN protein cellular localization and enzymatic activities by SIRT1-mediated deacetylation

UMMS Affiliation

Graduate School of Biomedical Sciences; Department of Cancer Biology and the Cancer Center

Date

1-22-2008

Document Type

Article

Medical Subject Headings

Acetylation; Aging, Premature; Animals; Caenorhabditis elegans; Cell Aging; Cell Line; Cell Nucleus; DNA Damage; Down-Regulation; E1A-Associated p300 Protein; Exodeoxyribonucleases; Genomic Instability; Humans; Longevity; Neoplasm Proteins; Neoplasms; Nuclear Proteins; RecQ Helicases; Sirtuins; Transcription Factors; Tumor Suppressor Protein p53; Tumor Suppressor Proteins

Disciplines

Life Sciences | Medicine and Health Sciences

Abstract

Werner syndrome is an autosomal recessive disorder associated with premature aging and cancer predisposition caused by mutations of the WRN gene. WRN is a member of the RecQ DNA helicase family with functions in maintaining genome stability. Sir2, an NAD-dependent histone deacetylase, has been proven to extend life span in yeast and Caenorhabditis elegans. Mammalian Sir2 (SIRT1) has also been found to regulate premature cellular senescence induced by the tumor suppressors PML and p53. SIRT1 plays an important role in cell survival promoted by calorie restriction. Here we show that SIRT1 interacts with WRN both in vitro and in vivo; this interaction is enhanced after DNA damage. WRN can be acetylated by acetyltransferase CBP/p300, and SIRT1 can deacetylate WRN both in vitro and in vivo. WRN acetylation decreases its helicase and exonuclease activities, and SIRT1 can reverse this effect. WRN acetylation alters its nuclear distribution. Down-regulation of SIRT1 reduces WRN translocation from nucleoplasm to nucleoli after DNA damage. These results suggest that SIRT1 regulates WRN-mediated cellular responses to DNA damage through deacetylation of WRN.

Rights and Permissions

Citation: J Biol Chem. 2008 Mar 21;283(12):7590-8. Epub 2008 Jan 17. Link to article on publisher's site

Related Resources

Link to Article in PubMed

Journal Title

The Journal of biological chemistry

PubMed ID

18203716