GSBS Student Publications

Student Author(s)

Michael Krashes

GSBS Program

Neuroscience

UMMS Affiliation

Department of Neurobiology; Waddell Lab; Graduate School of Biomedical Sciences, Neuroscience Program

Date

3-19-2008

Document Type

Article

Medical Subject Headings

Action Potentials; Animals; Animals, Genetically Modified; Appetitive Behavior; Behavior, Animal; Conditioning, Classical; Cyclic AMP; Cycloheximide; Drosophila; Drosophila Proteins; Food Deprivation; Memory; Mushroom Bodies; Mutation; Neurons; *Odors; Protein Biosynthesis; Protein Synthesis Inhibitors; Raphanus; Temperature; Time Factors

Disciplines

Behavioral Neurobiology

Abstract

In Drosophila, formation of aversive olfactory long-term memory (LTM) requires multiple training sessions pairing odor and electric shock punishment with rest intervals. In contrast, here we show that a single 2 min training session pairing odor with a more ethologically relevant sugar reinforcement forms long-term appetitive memory that lasts for days. Appetitive LTM has some mechanistic similarity to aversive LTM in that it can be disrupted by cycloheximide, the dCreb2-b transcriptional repressor, and the crammer and tequila LTM-specific mutations. However, appetitive LTM is completely disrupted by the radish mutation that apparently represents a distinct mechanistic phase of consolidated aversive memory. Furthermore, appetitive LTM requires activity in the dorsal paired medial neuron and mushroom body alpha'beta' neuron circuit during the first hour after training and mushroom body alphabeta neuron output during retrieval, suggesting that appetitive middle-term memory and LTM are mechanistically linked. Last, experiments feeding and/or starving flies after training reveals a critical motivational drive that enables appetitive LTM retrieval.

Rights and Permissions

Citation: J Neurosci. 2008 Mar 19;28(12):3103-13. Link to article on publisher's site

Comments

Publisher PDF posted as allowed by the publisher's author rights policy at http://www.jneurosci.org/site/misc/ifa_policies.xhtml#copyright.

Copyright of all material published in The Journal of Neuroscience remains with the authors. The authors grant the Society for Neuroscience an exclusive license to publish their work for the first 6 months. After 6 months the work becomes available to the public to copy, distribute, or display under a Creative Commons Attribution 4.0 International (CC BY 4.0) license.

Related Resources

Link to Article in PubMed

PubMed ID

18354013

Creative Commons License


This work is licensed under a Creative Commons Attribution 4.0 License.

Share

COinS
 
 

To view the content in your browser, please download Adobe Reader or, alternately,
you may Download the file to your hard drive.

NOTE: The latest versions of Adobe Reader do not support viewing PDF files within Firefox on Mac OS and if you are using a modern (Intel) Mac, there is no official plugin for viewing PDF files within the browser window.