GSBS Student Publications


Target accessibility dictates the potency of human RISC

GSBS Program

Biochemistry & Molecular Pharmacology

UMMS Affiliation

Graduate School of Biomedical Sciences; Department of Biochemistry and Molecular Pharmacology



Document Type


Medical Subject Headings

Base Sequence; Binding Sites; Catalysis; Cyclin-Dependent Kinase 9; HIV Long Terminal Repeat; Hela Cells; Humans; Kinetics; Molecular Sequence Data; Nucleic Acid Conformation; RNA, Viral; RNA-Induced Silencing Complex


Life Sciences | Medicine and Health Sciences


In this report, we examined the effect of increased target site access on activated human RNA-induced silencing complex (RISC(*)) catalysis. Kinetic studies revealed that siRNA-programmed RISC(*) cleaved target RNA with higher efficiencies when target site access was increased. These results provide evidence that target site access is linked to RISC(*) catalysis.

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Citation: Nat Struct Mol Biol. 2005 May;12(5):469-70. Epub 2005 Apr 24. Link to article on publisher's site

DOI of Published Version


Related Resources

Link to article in PubMed

Journal Title

Nature structural and molecular biology

PubMed ID