GSBS Student Publications

Title

Transcription activation in cells lacking TAFIIS

UMMS Affiliation

Graduate School of Biomedical Sciences; Howard Hughes Medical Institute; Program in Molecular Medicine

Date

9-12-1996

Document Type

Article

Medical Subject Headings

*Antigens, Neoplasm; Carrier Proteins; DNA-Binding Proteins; Gene Expression Regulation, Fungal; Heat-Shock Response; *Histocompatibility Antigens; Metallothionein; Molecular Sequence Data; Mutation; Saccharomyces cerevisiae; TATA Box; TATA-Box Binding Protein; Temperature; *Trans-Activation (Genetics); Transcription Factor TFIID; Transcription Factors; Transcription, Genetic

Disciplines

Life Sciences | Medicine and Health Sciences

Abstract

The general transcription factor TFIID is composed of the TATA-box-binding protein (TBP) and a set of TBP-associated factors (TAFIIs). In vitro, TAFIIs are required for activated transcription, and have been proposed to be obligatory targets of transcriptional activator proteins (activators)2. The function of TAFIIs has not been investigated systematically in vivo. A Saccharomyces cerevisiae TAFII complex (yTAFII complex) has been identified that shares functional and structural similarities with higher eukaryotic TFIID. In particular, most yTAFIIs are the homologue of a higher eukaryotic TAFII. Here we report that inactivation or depletion of six different yTAFIIs, including the core yTAFII, that contacts TBP, does not compromise transcriptional activation. We conclude that in vivo, activated transcription of many genes can occur in the absence of functional yTAFIIS, and that in these instances another transcription component(s) must be the target of the activator.

Rights and Permissions

Citation: Nature. 1996 Sep 12;383(6596):185-8. Link to article on publisher's site

DOI of Published Version

10.1038/383185a0

Related Resources

Link to Article in PubMed

Journal Title

Nature

PubMed ID

8774886