GSBS Student Publications

Title

Functional genomics reveals a family of eukaryotic oxidation protection genes

UMMS Affiliation

Graduate School of Biomedical Sciences; Department of Molecular Genetics and Microbiology

Date

12-13-2000

Document Type

Article

Medical Subject Headings

Amino Acid Sequence; Animals; Base Sequence; DNA, Complementary; Eukaryotic Cells; Humans; Hydrogen Peroxide; Molecular Sequence Data; Oxidation-Reduction; Proteins; Saccharomyces cerevisiae; Sequence Analysis, DNA

Disciplines

Life Sciences | Medicine and Health Sciences

Abstract

Reactive oxygen species (ROS) are toxic compounds produced by normal metabolic processes. Their reactivity with cellular components is a major stress for aerobic cells that results in lipid, protein, and DNA damage. ROS-mediated DNA damage contributes to spontaneous mutagenesis, and cells deficient in repair and protective mechanisms have elevated levels of spontaneous mutations. In Escherichia coli a large number of genes are involved in the repair of oxidative DNA damage and its prevention by detoxification of ROS. In humans, the genes required for these processes are not well defined. In this report we describe the human OXR1 (oxidation resistance) gene discovered in a search for human genes that function in protection against oxidative damage. OXR1 is a member of a conserved family of genes found in eukaryotes but not in prokaryotes. We also outline the procedures developed to identify human genes involved in the prevention and repair of oxidative damage that were used to identify the human OXR1 gene. This procedure makes use of the spontaneous mutator phenotype of E. coli oxidative repair-deficient mutants and identifies genes of interest by screening for antimutator activity resulting from cDNA expression.

Rights and Permissions

Citation: Proc Natl Acad Sci U S A. 2000 Dec 19;97(26):14530-5. Link to article on publisher's site

Related Resources

Link to Article in PubMed

Journal Title

Proceedings of the National Academy of Sciences of the United States of America

PubMed ID

11114193