GSBS Student Publications

Title

HAT activity is essential for CBP-1-dependent transcription and differentiation in Caenorhabditis elegans

UMMS Affiliation

Graduate School of Biomedical Sciences; Department of Pathology; Program in Molecular Medicine

Date

12-26-2001

Document Type

Article

Medical Subject Headings

Acetyltransferases; Alleles; Animals; Caenorhabditis elegans; *Caenorhabditis elegans Proteins; Cell Differentiation; Gene Deletion; Helminth Proteins; Histone Acetyltransferases; Mutation; Point Mutation; Promoter Regions (Genetics); Protein Structure, Tertiary; *Saccharomyces cerevisiae Proteins; *Transcription Factors; *Transcription, Genetic

Disciplines

Life Sciences | Medicine and Health Sciences

Abstract

The p300/CBP family of transcriptional coactivators possesses multiple functional domains, including a histone acetyltransferase (HAT) and several activation domains. A number of models have been proposed to account for their roles in transcriptional activation, including interactions with basal transcription machinery and chromatin remodeling. However, individual contributions of these domains to transcriptional activation and their significance in living organisms remain unclear. We addressed the importance of the HAT activity of CBP-1, the worm ortholog of p300/CBP, in Caenorhabditis elegans with three different and complementary approaches. These include allele-specific RNA-mediated interference (RNAi), genetic rescue and the use of a specific chemical inhibitor of the p300/CBP HAT. Our findings demonstrate that HAT activity is of primary importance for CBP-1 to regulate transcription and to promote differentiation during C. elegans embryogenesis.

Rights and Permissions

Citation: EMBO Rep. 2002 Jan;3(1):50-5. Epub 2001 Dec 19. Link to article on publisher's site

DOI of Published Version

10.1093/embo-reports/kvf006

Related Resources

Link to Article in PubMed

Journal Title

EMBO reports

PubMed ID

11751575