GSBS Student Publications

Title

DNA instructed displacement of histones H2A and H2B at an inducible promoter

UMMS Affiliation

Graduate School of Biomedical Sciences; Centre de Regulacio Genomica; Program in Molecular Medicine

Date

11-5-2004

Document Type

Article

Medical Subject Headings

Adenosine Triphosphate; Binding Sites; Cells, Cultured; Chromosomal Proteins, Non-Histone; DNA, Ribosomal; DNA-Binding Proteins; Electrophoretic Mobility Shift Assay; Histones; Humans; Mammary Tumor Virus, Mouse; NFI Transcription Factors; Nuclear Proteins; Nucleosomes; Progesterone; *Promoter Regions (Genetics); Receptors, Progesterone; Trans-Activation (Genetics); Transcription Factors

Disciplines

Life Sciences | Medicine and Health Sciences

Abstract

Regulation of gene expression requires dynamic changes in chromatin, but the nature of these changes is not well understood. Here, we show that progesterone treatment of cultured cells leads to recruitment of progesterone receptor (PR) and SWI/SNF-related complexes to Mouse Mammary Tumor Virus (MMTV) promoter, accompanied by displacement of histones H2A and H2B from the nucleosome containing the receptor binding sites, but not from adjacent nucleosomes. PR recruits SWI/SNF to MMTV nucleosomes in vitro and facilitates synergistic binding of receptors and nuclear factor 1 to the promoter. In nucleosomes assembled on MMTV or mouse rDNA promoter sequences, SWI/SNF catalyzes ATP-dependent sliding of the histone octamer followed only on the MMTV promoter by displacement of histones H2A and H2B. In MMTV nucleosome arrays, SWI/SNF displaces H2A and H2B from nucleosome B and not from the adjacent nucleosome. Thus, the outcome of nucleosome remodeling by SWI/SNF depends on DNA sequence.

Rights and Permissions

Citation: Mol Cell. 2004 Nov 5;16(3):439-52. Link to article on publisher's site

DOI of Published Version

10.1016/j.molcel.2004.10.025

Related Resources

Link to Article in PubMed

Journal Title

Molecular cell

PubMed ID

15525516