GSBS Student Publications

Title

High frequency of virus-specific interleukin-2-producing CD4(+) T cells and Th1 dominance during lymphocytic choriomeningitis virus infection

UMMS Affiliation

Graduate School of Biomedical Sciences; Program in Immunology and Virology; Department of Pathology

Date

2-7-2001

Document Type

Article

Medical Subject Headings

Acute Disease; Animals; CD4-Positive T-Lymphocytes; Cytokines; Immunologic Memory; Immunophenotyping; Interleukin-2; Intracellular Fluid; Lymphocytic Choriomeningitis; Mice; Mice, Inbred C57BL; Th1 Cells

Disciplines

Life Sciences | Medicine and Health Sciences

Abstract

Analysis of C57BL/6 mice acutely infected with lymphocytic choriomeningitis virus (LCMV) by using intracellular cytokine staining revealed a high frequency (2 to 10%) of CD4(+) T cells secreting the Th1-associated cytokines interleukin-2 (IL-2), gamma interferon (IFN-gamma), and tumor necrosis factor alpha, with no concomitant increase in the frequency of CD4(+) T cells secreting the Th2-associated cytokines IL-4, IL-5, and IL-10 following stimulation with viral peptides. In LCMV-infected C57BL/6 CD8(-/-) mice, more than 20% of the CD4(+) T cells secreted IFN-gamma after viral peptide stimulation, whereas less than 1% of the CD4(+) T cells secreted IL-4 under these same conditions. Mice persistently infected with a high dose of LCMV clone 13 also generated a virtually exclusive Th1 response. Thus, LCMV induces a much more profound virus-specific CD4(+) T-cell response than previously recognized, and it is dramatically skewed to a Th1 phenotype.

Rights and Permissions

Citation: J Virol. 2000 May;74(9):4429-32.

Related Resources

Link to Article in PubMed

Journal Title

Journal of virology

PubMed ID

10756059