GSBS Student Publications

Title

The CD45RB-associated epitope defined by monoclonal antibody CZ-1 is an activation and memory marker for mouse CD4 T cells

Student Author(s)

Steven Michael Varga

GSBS Program

Immunology & Virology Program

UMMS Affiliation

Department of Pathology

Date

1-10-1996

Document Type

Article

Medical Subject Headings

Animals; Antibodies, Monoclonal; Antigens, CD45; CD4-Positive T-Lymphocytes; *Epitopes; *Immunologic Memory; Male; Mice; Mice, Inbred C57BL; Rats

Disciplines

Immunology and Infectious Disease

Abstract

Monoclonal antibody CZ-1 defines a novel sialic acid-dependent CD45RB-associated epitope. The CZ-1 antigen is expressed on the subpopulation of CD4 T cells that proliferate in response to IL-2. Because IL-2 responsiveness often denotes T cell activation, we examined the expression of the CZ-1 antigen on CD4 T cells taken from mice at various times during an infection with lymphocytic choriomeningitis virus (LCMV). The blast-sized CD4 T cells at Day 6 postinfection were CZ-1+. Further cell surface phenotyping showed that those blast cells activated at Day 6 postinfection were CD45RBdimPgp-1brightMEL-14-. This contrasts with the CZ-1-CD45RBbrightPgp-1bright/dimMEL-14+ resting cell population prior to infection. To determine if memory CD4 T cells continued to express the CZ-1 epitope long after resolution of the LCMV infection, CD4+CZ-1+ and CD4+CZ-1- populations were purified by cell sorting and placed in an in vitro proliferation assay with LCMV-infected antigen-presenting cells. It was found that the CD4+CZ-1+ population contained virtually all of the virus-specific memory. The CZ-1 epitope is therefore both an activation and a memory marker for murine CD4 T cells.

Rights and Permissions

Citation: Cell Immunol. 1996 Jan 10;167(1):56-62. Link to article on publisher's site

Related Resources

Link to Article in PubMed

Journal Title

Cellular immunology

PubMed ID

8548845