CDP/cut is the DNA-binding subunit of histone gene transcription factor HiNF-D: a mechanism for gene regulation at the G1/S phase cell cycle transition point independent of transcription factor E2F
Authors
Van Wijnen, Andre J.van Gurp, Maria F.
De Ridder, Marleen C.
Tufarelli, Cristina
Last, Thomas J.
Birnbaum, Mark J.
Vaughan, Patricia S.
Giordano, Antonio
Wilhelm, Krek
Neufeld, Ellis J.
Stein, Janet L.
Stein, Gary S.
Document Type
Journal ArticlePublication Date
1996-10-15Keywords
Base Sequence; Binding Sites; *Carrier Proteins; *Cell Cycle; *Cell Cycle Proteins; Cell Nucleus; Consensus Sequence; DNA Footprinting; DNA Replication; DNA-Binding Proteins; E2F Transcription Factors; G1 Phase; Hela Cells; Histones; Homeodomain Proteins; Humans; Molecular Sequence Data; Mutagenesis, Site-Directed; Nuclear Proteins; *Promoter Regions (Genetics); Recombinant Fusion Proteins; Repressor Proteins; Restriction Mapping; S Phase; Transcription Factor DP1; Transcription FactorsLife Sciences
Medicine and Health Sciences
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Show full item recordAbstract
Transcription of the genes for the human histone proteins H4, H3, H2A, H2B, and H1 is activated at the G1/S phase transition of the cell cycle. We have previously shown that the promoter complex HiNF-D, which interacts with cell cycle control elements in multiple histone genes, contains the key cell cycle factors cyclin A, CDC2, and a retinoblastoma (pRB) protein-related protein. However, an intrinsic DNA-binding subunit for HiNF-D was not identified. Many genes that are up-regulated at the G1/S phase boundary are controlled by E2F, a transcription factor that associates with cyclin-, cyclin-dependent kinase-, and pRB-related proteins. Using gel-shift immunoassays, DNase I protection, and oligonucleotide competition analyses, we show that the homeodomain protein CDP/cut, not E2F, is the DNA-binding subunit of the HiNF-D complex. The HiNF-D (CDP/cut) complex with the H4 promoter is immunoreactive with antibodies against CDP/cut and pRB but not p107, whereas the CDP/cut complex with a nonhistone promoter (gp91-phox) reacts only with CDP and p107 antibodies. Thus, CDP/cut complexes at different gene promoters can associate with distinct pRB-related proteins. Transient coexpression assays show that CDP/cut modulates H4 promoter activity via the HiNF-D-binding site. Hence, DNA replication-dependent histone H4 genes are regulated by an E2F-independent mechanism involving a complex of CDP/cut with cyclin A/CDC2/ RB-related proteins.Source
Proc Natl Acad Sci U S A. 1996 Oct 15;93(21):11516-21.
DOI
10.1073/pnas.93.21.11516Permanent Link to this Item
http://hdl.handle.net/20.500.14038/32740PubMed ID
8876167Related Resources
ae974a485f413a2113503eed53cd6c53
10.1073/pnas.93.21.11516
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