Protein/DNA interactions involving ATF/AP1-, CCAAT-, and HiNF-D-related factors in the human H3-ST519 histone promoter: cross-competition with transcription regulatory sites in cell cycle controlled H4 and H1 histone genes
Graduate School of Biomedical Sciences; Department of Cell Biology
Medical Subject Headings
Activating Transcription Factors; Base Sequence; Binding Sites; Binding, Competitive; Blood Proteins; CCAAT-Enhancer-Binding Proteins; Cell Cycle; DNA; DNA Fingerprinting; DNA Probes; DNA-Binding Proteins; Deoxyribonuclease I; *Gene Expression Regulation; Histones; Humans; Methylation; Molecular Sequence Data; *Promoter Regions (Genetics); Sulfuric Acid Esters; Transcription Factors; Transcription, Genetic
Life Sciences | Medicine and Health Sciences
Protein/DNA interactions of the H3-ST519 histone gene promoter were analyzed in vitro. Using several assays for sequence specificity, we established binding sites for ATF/AP1-, CCAAT-, and HiNF-D related DNA binding proteins. These binding sites correlate with two genomic protein/DNA interaction domains previously established for this gene. We show that each of these protein/DNA interactions has a counterpart in other histone genes: H3-ST519 and H4-F0108 histone genes interact with ATF- and HiNF-D related binding activities, whereas H3-ST519 and H1-FNC16 histone genes interact with the same CCAAT-box binding activity. These factors may function in regulatory coupling of the expression of different histone gene classes. We discuss these results within the context of established and putative protein/DNA interaction sites in mammalian histone genes. This model suggests that heterogeneous permutations of protein/DNA interaction elements, which involve both general and cell cycle regulated DNA binding proteins, may govern the cellular competency to express and coordinately control multiple distinct histone genes.
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Citation: J Cell Biochem. 1991 Dec;47(4):337-51. Link to article on publisher's site
Journal of cellular biochemistry
Van Wijnen, Andre J.; Lian, Jane B.; Stein, Janet L.; and Stein, Gary S., "Protein/DNA interactions involving ATF/AP1-, CCAAT-, and HiNF-D-related factors in the human H3-ST519 histone promoter: cross-competition with transcription regulatory sites in cell cycle controlled H4 and H1 histone genes" (1991). GSBS Student Publications. 1288.