Involvement of the cell cycle-regulated nuclear factor HiNF-D in cell growth control of a human H4 histone gene during hepatic development in transgenic mice
Authors
Van Wijnen, Andre J.Choi, Theodore K.
Owen, T. A.
Wright, Kenneth Lynn
Lian, Jane B.
Jaenisch, Rudolf
Stein, Janet L.
Stein, Gary S.
Document Type
Journal ArticlePublication Date
1991-03-15Keywords
Aging; Animals; Brain; *Cell Cycle; Cell Division; Cells, Cultured; DNA Replication; Histones; Humans; Liver; Mice; Mice, Transgenic; Nuclear Proteins; Plasmids; RNA, Messenger; *Transcription, GeneticLife Sciences
Medicine and Health Sciences
Metadata
Show full item recordAbstract
Regulation of the cell cycle-controlled histone gene promoter factor HiNF-D was examined in vivo. Proliferative activity was measured by DNA replication-dependent histone mRNA levels, and HiNF-D binding activity was found to correlate with cell proliferation in most tissues. Furthermore, HiNF-D is down-regulated during hepatic development, reflecting the onset of differentiation and quiescence. The contribution of transcription to histone gene expression was directly addressed in transgenic mice by using a set of fusion constructs containing a human H4 histone gene promoter linked to three different genes. Transgene expression in both fetal and adult mice paralleled endogenous mouse histone mRNA levels in most tissues, consistent with this promoter conferring developmental, cell growth-related transcriptional regulation. Our results suggest that HiNF-D is stringently regulated in vivo in relation to cell growth and support a primary role for HiNF-D in the proliferation-specific expression of H4 histone genes in the intact animal. Further, the data presented here provide an example in which apparent tissue specificity of gene expression reflects the proliferative state of various tissues and demonstrate that multiple levels of histone gene regulation are operative in vivo.Source
Proc Natl Acad Sci U S A. 1991 Mar 15;88(6):2573-7.
DOI
10.1073/pnas.88.6.2573Permanent Link to this Item
http://hdl.handle.net/20.500.14038/32730PubMed ID
2006193Related Resources
ae974a485f413a2113503eed53cd6c53
10.1073/pnas.88.6.2573