GSBS Student Publications

Title

DNA immunization: effect of secretion of DNA-expressed hemagglutinins on antibody responses

UMMS Affiliation

Graduate School of Biomedical Sciences; Department of Medical Microbiology

Date

12-2-1999

Document Type

Article

Medical Subject Headings

Animals; Antibodies, Viral; Enzyme-Linked Immunosorbent Assay; Female; Hemagglutinin Glycoproteins, Influenza Virus; Hemagglutinins, Viral; Humans; Immunoglobulin G; Interleukin-4; Mice; Mice, Inbred BALB C; Mice, Knockout; Plasmids; T-Lymphocytes, Helper-Inducer; *Vaccines, DNA

Disciplines

Life Sciences | Medicine and Health Sciences

Abstract

DNA vaccines expressing plasma membrane and secreted forms of the influenza and measles virus hemagglutinins (HAs) have been used to evaluate the effect of secretion on DNA-raised antibody responses. At low doses of DNA, the plasma membrane form of the influenza virus HA raised higher titers of antibody than the secreted form. The isotype of the DNA-raised antibodies depended on both the method of DNA delivery and the form of the expressed antigen. Following intramuscular injections, DNAs expressing membrane bound forms of the influenza and measles HAs raised predominantly IgG2a. By contrast, DNAs expressing the secreted from of the two HAs as well as another secreted protein, human growth hormone, raised predominantly IgG1. Gene gun delivery resulted in predominantly IgG1 antibody responses for both secreted and membrane bound forms of the hemagglutinins. The raising of predominantly IgG1 by i.m. delivery of the secreted form of the influenza hemagglutinin was IL-4 dependent suggesting that a T-helper 2-biased immune response had been raised.

Rights and Permissions

Citation: Vaccine. 1999 Dec 10;18(9-10):805-14.

Related Resources

Link to Article in PubMed

Journal Title

Vaccine

PubMed ID

10580193