GSBS Student Publications

Title

Soluble CD8 during T cell activation

UMMS Affiliation

Graduate School of Biomedical Sciences; Department of Pediatrics

Date

4-1-1989

Document Type

Article

Medical Subject Headings

Adolescent; Adult; Antibodies, Monoclonal; Antigens, Differentiation, T-Lymphocyte; purification; Cell Line; Humans; Infectious Mononucleosis; Kinetics; *Lymphocyte Activation; T-Lymphocytes

Disciplines

Life Sciences | Medicine and Health Sciences

Abstract

The CD8 Ag has long been used as a surface marker for the identification of cytotoxic and suppressor cells. Recently CD8-positive cells have been shown to release a soluble form of the CD8 Ag. We have devised a sandwich monoclonal enzyme immunoassay for the quantitation of this released CD8. Soluble CD8 was released in response to lymphocyte activation. In vitro, PHA or anti-CD3 mAb-mediated T cell activation led to release of CD8 into the culture supernatant. In vivo, serum from patients with EBV-induced infectious mononucleosis (IM), a disease associated with intense CD8+ T cell activation, demonstrated elevations in soluble CD8 (7939 U/ml, day 0) compared to serum from normal controls (289 U/ml). Levels of soluble CD8 correlated (r = 0.82, p less than 0.001) with the increased percentage of CD8+/HLA-DR+ (activated CD8+ T cells) observed in acute IM. Sequential analysis of serum during the course of IM shows that soluble CD8 levels parallel the decline in CD8+/HLA-DR+ cells that occurs with the resolution of the disease. These data suggest that released CD8 may be of value in monitoring the involvement of CD8+ T cells in response to a pathologic event. The functional role of the released CD8 molecule will require further investigation.

Rights and Permissions

Citation: J Immunol. 1989 Apr 1;142(7):2230-6.

Related Resources

Link to Article in PubMed

Journal Title

Journal of immunology (Baltimore, Md. : 1950)

PubMed ID

2784457