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Authors
Tomari, YukihideDu, Tingting
Haley, Benjamin
Schwarz, Dianne S.
Bennett, Ryan
Cook, Heather A.
Koppetsch, Birgit S.
Theurkauf, William E.
Zamore, Phillip D.
UMass Chan Affiliations
Program in Molecular MedicineDepartment of Biochemistry and Molecular Pharmacology
Graduate School of Biomedical Sciences
Document Type
Journal ArticlePublication Date
2004-03-24Keywords
Animals; Body Patterning; Cell Differentiation; Drosophila Proteins; Drosophila melanogaster; Embryo, Nonmammalian; Female; Germ Cells; Insect Proteins; Male; Mutation; Oocytes; Protein Kinases; RNA Helicases; RNA Interference; RNA, Small Interfering; RNA-Induced Silencing ComplexLife Sciences
Medicine and Health Sciences
Metadata
Show full item recordAbstract
The putative RNA helicase, Armitage (Armi), is required to repress oskar translation in Drosophila oocytes; armi mutant females are sterile and armi mutations disrupt anteroposterior and dorsoventral patterning. Here, we show that armi is required for RNAi. armi mutant male germ cells fail to silence Stellate, a gene regulated endogenously by RNAi, and lysates from armi mutant ovaries are defective for RNAi in vitro. Native gel analysis of protein-siRNA complexes in wild-type and armi mutant ovary lysates suggests that armi mutants support early steps in the RNAi pathway but are defective in the production of active RNA-induced silencing complex (RISC), which mediates target RNA destruction in RNAi. Our results suggest that armi is required for RISC maturation.Source
Cell. 2004 Mar 19;116(6):831-41.
DOI
10.1016/S0092-8674(04)00218-1Permanent Link to this Item
http://hdl.handle.net/20.500.14038/32698PubMed ID
15035985Related Resources
ae974a485f413a2113503eed53cd6c53
10.1016/S0092-8674(04)00218-1