FMRI of brain activation in a genetic rat model of absence seizures
Graduate School of Biomedical Sciences; Center for Comparative Neuroimaging; Department of Psychiatry
Medical Subject Headings
Animals; Brain; Brain Mapping; Cerebral Cortex; Disease Models, Animal; *Electroencephalography; Epilepsy, Absence; *Magnetic Resonance Imaging; Male; Neural Pathways; Oxygen; Rats; Rats, Wistar; Thalamic Nuclei
Life Sciences | Medicine and Health Sciences
PURPOSE: EEG-triggered functional magnetic resonance imaging (fMRI) was used to identify areas of brain activation during spontaneous spike-and-wave discharges (SWDs) in an epileptic rat strain under awake conditions.
METHODS: Spontaneous absence seizures from 10 WAG/Rij rats were imaged by using T2*-weighted echo planar imaging at 4.7 Tesla. fMRI of the blood-oxygenation-level-dependent (BOLD) signal was triggered based on EEG recordings during imaging. Images obtained during spontaneous SWDs were compared with baseline images.
RESULTS: Significant positive BOLD signal changes were apparent in several areas of the cortex and several important nuclei of the thalamus. In addition, no negative BOLD signal was found in any brain area.
CONCLUSIONS: We have shown that EEG-triggered BOLD fMRI can be used to detect cortical and thalamic activation related to the spontaneous SWDs that characterize absence seizures in awake WAG/Rij rats. These results draw an anatomic correlation between areas in which increased BOLD signal is found and those in which SWDs have been recorded. In addition, no negative BOLD signal was found to be associated with these spontaneous SWDs. We also demonstrated the technical feasibility of using EEG-triggered fMRI in a genetic rat model of absence seizure.
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Citation: Epilepsia. 2004 Jun;45(6):576-82. Link to article on publisher's site