Role of different lymphoid tissues in the initiation and maintenance of DNA-raised antibody responses to the influenza virus H1 glycoprotein
Biochemistry & Molecular Pharmacology
Graduate School of Biomedical Sciences
Medical Subject Headings
Animals; Antibodies, Viral; Biolistics; DNA, Viral; Hemagglutinin Glycoproteins, Influenza Virus; Humans; Immunoglobulin G; Influenza A virus; Influenza Vaccines; Influenza, Human; Lymphoid Tissue; Mice; Mice, Inbred BALB C; Time Factors; Vaccines, DNA
Life Sciences | Medicine and Health Sciences
Antibody responses in mice immunized by a single gene gun inoculation of plasmid expressing the influenza virus H1 hemagglutinin and in mice immunized by a sublethal H1 influenza virus infection have been compared. Both immunizations raised long-lived serum responses that were associated with the localization of antibody-secreting cells (ASC) to the bone marrow. However, the kinetics of these responses were 4 to 8 weeks slower in the DNA-immunized than in the infection-primed mice. Following a gene gun booster, the presence of ASC in the inguinal lymph nodes, but not in other lymph nodes, revealed gene gun responses being initiated in the nodes that drain the skin target site. Both pre- and postchallenge, the DNA-immunized mice had 5- to 10-times-lower levels of antibody and ASC than the infection-primed mice.
Rights and Permissions
Citation: J Virol. 1996 Dec;70(12):9074-8.
Journal of virology
Boyle, Christine Margaret; Morin, Merribeth J.; Webster, Robert G.; and Robinson, Harriet L., "Role of different lymphoid tissues in the initiation and maintenance of DNA-raised antibody responses to the influenza virus H1 glycoprotein" (1996). GSBS Student Publications. 122.