The rde-1 gene, RNA interference, and transposon silencing in C. elegans
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Authors
Tabara, HiroakiSarkissian, Madathia
Kelly, William G.
Fleenor, Jamie
Grishok, Alla
Timmons, Lisa
Fire, Andrew Z.
Mello, Craig C.
UMass Chan Affiliations
Program in Molecular MedicineDepartment of Cell Biology
Graduate School of Biomedical Sciences
Document Type
Journal ArticlePublication Date
1999-10-27Keywords
Amino Acid Sequence; Animals; Animals, Genetically Modified; Caenorhabditis elegans; *Caenorhabditis elegans Proteins; Chromosomes, Artificial, Yeast; Cosmids; DNA Transposable Elements; Green Fluorescent Proteins; Helminth Proteins; Homozygote; Luminescent Proteins; Molecular Sequence Data; *Mutation; RNA, Double-Stranded; RNA, Helminth; Sequence Alignment; Sequence Homology, Amino AcidLife Sciences
Medicine and Health Sciences
Metadata
Show full item recordAbstract
Double-stranded (ds) RNA can induce sequence-specific inhibition of gene function in several organisms. However, both the mechanism and the physiological role of the interference process remain mysterious. In order to study the interference process, we have selected C. elegans mutants resistant to dsRNA-mediated interference (RNAi). Two loci, rde-1 and rde-4, are defined by mutants strongly resistant to RNAi but with no obvious defects in growth or development. We show that rde-1 is a member of the piwi/sting/argonaute/zwille/eIF2C gene family conserved from plants to vertebrates. Interestingly, several, but not all, RNAi-deficient strains exhibit mobilization of the endogenous transposons. We discuss implications for the mechanism of RNAi and the possibility that one natural function of RNAi is transposon silencing.Source
Cell. 1999 Oct 15;99(2):123-32.
DOI
10.1016/S0092-8674(00)81644-XPermanent Link to this Item
http://hdl.handle.net/20.500.14038/32660PubMed ID
10535731Related Resources
ae974a485f413a2113503eed53cd6c53
10.1016/S0092-8674(00)81644-X