GSBS Student Publications


Functional delineation of three groups of the ATP-dependent family of chromatin remodeling enzymes

GSBS Program

Biochemistry & Molecular Pharmacology

UMMS Affiliation

Graduate School of Biomedical Sciences; Department of Cell Biology; Program in Molecular Medicine



Document Type


Medical Subject Headings

Adenosine Triphosphatases; Adenosine Triphosphate; Chromatin; Kinetics; Protein Conformation; Trans-Activators


Life Sciences | Medicine and Health Sciences


ATP-dependent chromatin remodeling enzymes antagonize the inhibitory effects of chromatin. We compare six different remodeling complexes: ySWI/SNF, yRSC, hSWI/SNF, xMi-2, dCHRAC, and dNURF. We find that each complex uses similar amounts of ATP to remodel nucleosomal arrays at nearly identical rates. We also perform assays with arrays reconstituted with hyperacetylated or trypsinized histones and isolated histone (H3/H4)(2) tetramers. The results define three groups of the ATP-dependent family of remodeling enzymes. In addition we investigate the ability of an acidic activator to recruit remodeling complexes to nucleosomal arrays. We propose that ATP-dependent chromatin remodeling enzymes share a common reaction mechanism and that a key distinction between complexes is in their mode of regulation or recruitment.

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Citation: J Biol Chem. 2000 Jun 23;275(25):18864-70. Link to article on publisher's site

Related Resources

Link to article in PubMed

PubMed ID