GSBS Student Publications

Title

Regulation of transcription-factor activity during growth and differentiation: involvement of the nuclear matrix in concentration and localization of promoter binding proteins

UMMS Affiliation

Graduate School of Biomedical Sciences; Department of Cell Biology; Department of Medicine, Diabetes Division

Date

12-1-1991

Document Type

Article

Medical Subject Headings

Animals; Cell Differentiation; DNA Replication; DNA-Binding Proteins; Gene Expression Regulation; Nuclear Matrix; Promoter Regions (Genetics); Transcription Factors; Transcription, Genetic

Disciplines

Life Sciences | Medicine and Health Sciences

Abstract

Several lines of evidence are presented which support involvement of the nuclear matrix in regulating the transcription of two genes, histone and osteocalcin, that are reciprocally expressed during development of the osteoblast phenotype. In the 5' regulatory region of an H4 histone gene, which is expressed in proliferating osteoblasts early during the developmental/differentiation sequence, a dual role is proposed for the nuclear matrix binding domain designated NMP-1 (-589 to -730 upstream from the transcription start site). In addition to functioning as a nuclear matrix attachment site, the sequences contribute to the upregulation of histone gene transcription, potentially facilitated by concentration and localization of an 84kD ATF DNA binding protein. A homologous nuclear matrix binding domain was identified in the promoter of the osteocalcin gene, which is expressed in mature osteoblasts in an extracellular matrix undergoing mineralization. The NMP binding domain in the osteocalcin gene promoter resides contiguous to the vitamin D responsive element. Together with gene and transcription factor localization, a model is proposed whereby nuclear matrix-associated structural constraints on conformation of the osteocalcin gene promoter facilitates vitamin D responsiveness mediated by cooperativity at multiple regulatory elements.

Rights and Permissions

Citation: J Cell Biochem. 1991 Dec;47(4):300-5. Link to article on publisher's site

DOI of Published Version

10.1002/jcb.240470403

Related Resources

Link to Article in PubMed

Journal Title

Journal of cellular biochemistry

PubMed ID

1795014