GSBS Student Publications


Synthesis and biochemical study of N2-(p-n-butylphenyl)-2'-deoxyguanosine 5'-(alpha,beta-imido)triphosphate (BuPdGMPNHPP): a non-substrate inhibitor of B family DNA polymerases

GSBS Program

Biochemistry & Molecular Pharmacology

UMMS Affiliation

Graduate School of Biomedical Sciences; Department of Pharmacology and Molecular Toxicology



Document Type


Medical Subject Headings

DNA Polymerase beta; Deoxyguanine Nucleotides; Enzyme Inhibitors; Guanylyl Imidodiphosphate; Humans


Life Sciences | Medicine and Health Sciences


BuPdGMPNHPP was synthesized and assayed as a non-incorporable inhibitor of B family DNA polymerases. The derivative was synthesized by preparation of the imidophosphorane of BuPdG followed by reaction with orthophosphate using the imidazolide method. BuPdGMPNHPP inhibited human DNA polymerase alpha and T4 DNA polymerase 10 and 3.5-times more potently than BuPdGTP, respectively, and was not a substrate for either enzyme. BuPdGMPNHPP acts as an active site affinity probe that could find use in co-crystallization trials of B family DNA polymerases.

Rights and Permissions

Citation: Nucleosides Nucleotides. 1998 Aug;17(8):1505-13.

Related Resources

Link to Article in PubMed

Journal Title

Nucleosides and nucleotides

PubMed ID