Title

Sensitivity of bacteriophage RB69 DNA polymerase to N2-(p-n-butylphenyl)-2'-deoxyguanosine nucleotides

GSBS Program

Biochemistry & Molecular Pharmacology

UMMS Affiliation

Graduate School of Biomedical Sciences; Department of Pharmacology and Molecular Toxicology

Date

1-5-2000

Document Type

Article

Medical Subject Headings

Bacteriophage T4; Bacteriophages; Crystallography, X-Ray; DNA-Directed DNA Polymerase; Deoxyguanine Nucleotides; Enzyme Inhibitors

Disciplines

Life Sciences | Medicine and Health Sciences

Abstract

The response of bacteriophage RB69 DNA polymerase to N2-(p-n-butylphenyl)-2'-deoxyguanosine 5'-triphosphate (BuPdGTP), related nucleotides and non-nucleoside inhibitors was measured and compared to values obtained for the closely related DNA polymerase from bacteriophage T4. Both enzymes showed similar responses to inhibitors in terms of Ki values and the ability to utilize BuPdGTP as a substrate. These results provide the relevance of using the recent crystal structure of RB69 DNA polymerase for analysis of BuPdGTP/B family DNA polymerase interactions.

Rights and Permissions

Citation: Nucleosides Nucleotides. 1999 Oct;18(10):2193-9.

Related Resources

Link to Article in PubMed

PubMed ID

10616725