Title
Sensitivity of bacteriophage RB69 DNA polymerase to N2-(p-n-butylphenyl)-2'-deoxyguanosine nucleotides
GSBS Program
Biochemistry & Molecular Pharmacology
UMMS Affiliation
Graduate School of Biomedical Sciences; Department of Pharmacology and Molecular Toxicology
Date
1-5-2000
Document Type
Article
Medical Subject Headings
Bacteriophage T4; Bacteriophages; Crystallography, X-Ray; DNA-Directed DNA Polymerase; Deoxyguanine Nucleotides; Enzyme Inhibitors
Disciplines
Life Sciences | Medicine and Health Sciences
Abstract
The response of bacteriophage RB69 DNA polymerase to N2-(p-n-butylphenyl)-2'-deoxyguanosine 5'-triphosphate (BuPdGTP), related nucleotides and non-nucleoside inhibitors was measured and compared to values obtained for the closely related DNA polymerase from bacteriophage T4. Both enzymes showed similar responses to inhibitors in terms of Ki values and the ability to utilize BuPdGTP as a substrate. These results provide the relevance of using the recent crystal structure of RB69 DNA polymerase for analysis of BuPdGTP/B family DNA polymerase interactions.
Rights and Permissions
Citation: Nucleosides Nucleotides. 1999 Oct;18(10):2193-9.