Sensitivity of bacteriophage RB69 DNA polymerase to N2-(p-n-butylphenyl)-2'-deoxyguanosine nucleotides
UMass Chan Affiliations
Department of Pharmacology and Molecular ToxicologyGraduate School of Biomedical Sciences
Document Type
Journal ArticlePublication Date
2000-01-05Keywords
Bacteriophage T4; Bacteriophages; Crystallography, X-Ray; DNA-Directed DNA Polymerase; Deoxyguanine Nucleotides; Enzyme InhibitorsLife Sciences
Medicine and Health Sciences
Metadata
Show full item recordAbstract
The response of bacteriophage RB69 DNA polymerase to N2-(p-n-butylphenyl)-2'-deoxyguanosine 5'-triphosphate (BuPdGTP), related nucleotides and non-nucleoside inhibitors was measured and compared to values obtained for the closely related DNA polymerase from bacteriophage T4. Both enzymes showed similar responses to inhibitors in terms of Ki values and the ability to utilize BuPdGTP as a substrate. These results provide the relevance of using the recent crystal structure of RB69 DNA polymerase for analysis of BuPdGTP/B family DNA polymerase interactions.Source
Nucleosides Nucleotides. 1999 Oct;18(10):2193-9.
DOI
10.1080/07328319908044875Permanent Link to this Item
http://hdl.handle.net/20.500.14038/32598PubMed ID
10616725Related Resources
ae974a485f413a2113503eed53cd6c53
10.1080/07328319908044875