GSBS Student Publications

Title

Differences in synthesis of membrane proteins by leukemic cells from spleen and peripheral blood indicate distinct subsets of malignant cells in a patient with prolymphocytic leukemia

UMMS Affiliation

Graduate School of Biomedical Sciences; Department of Pharmacology; Department of Pathology; Department of Pediatrics

Date

11-1-1981

Document Type

Article

Medical Subject Headings

Aged; Blood Cells; Cell Transformation, Neoplastic; Cells, Cultured; Electrophoresis, Polyacrylamide Gel; Humans; Immunoglobulin M; Leukemia, Lymphoid; Lymph Nodes; Male; Membrane Proteins; Molecular Weight; Spleen

Disciplines

Life Sciences | Medicine and Health Sciences

Abstract

Morphological and biochemical differences were demonstrated between prolymphocytic leukemia cells obtained from the spleen and peripheral blood of one patient. Peripheral blood prolymphocytes had consistently smaller nuclear-cytoplasmic ratios than did splenic prolymphocytes. Percoll gradient-purified prolymphocytes from the spleen synthesized abundant amounts of some membrane proteins which were hardly expressed by peripheral blood prolymphocytes. Peripheral blood prolymphocytes did not change their expression of membrane proteins during three days in culture. These findings are consistent with the view that prolymphocytic leukemia cells from the spleen exist, on the average, at an earlier stage of differentiation than do circulating leukemic cells, and that peripheral blood leukemic cells are frozen at a specific phase of differentiation.

Rights and Permissions

Citation: Am J Hematol. 1981 Nov;11(3):261-70.

Related Resources

Link to Article in PubMed

Journal Title

American journal of hematology

PubMed ID

6947689