GSBS Student Publications

Title

Genetic analysis of the 9.5-kilobase virulence plasmid of Yersinia pestis

UMMS Affiliation

Graduate School of Biomedical Sciences; Department of Molecular Genetics and Microbiology

Date

10-1-1988

Document Type

Article

Medical Subject Headings

Bacterial Outer Membrane Proteins; Bacterial Proteins; Bacteriocins; Chromosome Mapping; Cloning, Molecular; Coagulase; DNA Restriction Enzymes; Genes; *Genes, Bacterial; Peptide Hydrolases; *Plasmids; Plasminogen Activators; Yersinia pestis

Disciplines

Life Sciences | Medicine and Health Sciences

Abstract

The 9.5-kilobase plasmid of Yersinia pestis determines plasminogen activator, coagulase, pesticin, and pesticin immunity activities. We have mapped and cloned the loci encoding these activities and demonstrated that both plasminogen activator and coagulase were determined by the same gene, designated pla. The primary translation product of this gene (38 kilodaltons [kDa]) was processed in two sequential steps to produce peptides of 37 and 35 kDa. The first step in this processing occurred rapidly and probably cotranslationally and was blocked when protein export was inhibited. The second step was much slower and resulted in the presence of both the 37- and 35-kDa species in significant quantities. We also showed that the plasmid had a polA-dependent replicon and identified the region that contained its origin of replication and incompatibility functions.

Rights and Permissions

Citation: Infect Immun. 1988 Oct;56(10):2743-8.

Related Resources

Link to Article in PubMed

Journal Title

Infection and immunity

PubMed ID

2843470